Confidence and integrity are critical in the physical and chemical analyses of tissues and living cells. However, many of the probes targeting biological markers for confocal spectroscopy affect cells’ molecular identity. Hence, we combined photonics with electrical analysis in an assisted laser impedance spectroscopy facility and applied it to characterize two breast cancer cell lines (BT-474 and MCF-7) and lymphocytes (as a normal control). The setup comprised a sample holder with a ∼15 000 cell capacity fitted with two isolated conducting electrodes arranged concentrically and connected to an impedance analyser with a 20 Hz–1 MHz sweeping frequency. Capacitive transconductance measurements showed bands at 3491, 3494 and 3470 Hz corresponding to the BT-474, MCF-7, and lymphocytes, respectively. Under photonic stimulation by a 532 nm laser, these dark reference bands shifted to 3518, 3566 and 3674 Hz, respectively, reflecting optical transitions favouring ionic transport in the cells. Based on the experimental Nyquist diagrams and taking into account the roughness nature of the cell membrane, a constant phase element (CPE) was introduced in the circuit. The CPE was explained through a fractional parameter, α, based on fractional calculus. Results showed that, under photonic stimulation, α is less than , and the minimum change of series and membrane resistances are about 28.95% and 58.88%, respectively.

信心和完整性对​​于组织和活细胞的物理和化学分析至关重要。但是，许多针对生物标记物的共聚焦光谱探针会影响细胞的分子身份。因此，我们在辅助的激光阻抗谱仪中将光子学与电学分析相结合，并将其应用于表征两种乳腺癌细胞系（BT-474和MCF-7）和淋巴细胞（作为正常对照）的特征。该装置包括一个样品池，样品池容量约为15,000，装有两个同心排列的隔离导电电极，并连接到扫描频率为20 Hz–1 MHz的阻抗分析仪。电容跨导测量显示分别对应于BT-474，MCF-7和淋巴细胞的3491、3494和3470 Hz频带。在532 nm激光的光子刺激下，这些暗参考带分别移至3518、3566和3674 Hz，反映出有利于离子在细胞中转运的光学跃迁。根据实验的奈奎斯特图，并考虑到细胞膜的粗糙度，在电路中引入了恒定相元件（CPE）。通过分数参数解释了CPE，α，基于分数演算。结果表明，在光子刺激下，α小于，串联电阻和膜电阻的最小变化分别约为28.95％和58.88％。