Product quality control is a prerequisite for ensuring safety, effectiveness, and stability. However, because of the different strain species and fermentation processes, there was a significant difference in quality. As a result, they should be clearly distinguished in clinical use. Among them, the fermentation process is critical to achieving consistent product quality. This study aims to introduce near-infrared spectroscopy analysis technology into the production process of fermented Cordyceps powder, including strain culture, strain passage, strain fermentation, strain filtration, strain drying, strain pulverizing, and strain mixing. First, high performance liquid chromatography (HPLC) was used to measure the total nucleosides content in the production process of 30 batches of fermented Cordyceps powder, including uracil, uridine, adenine, guanosine, adenosine, and the process stability and interbatch consistency were analyzed with traditional Chinese medicine (TCM) fingerprinting, followed by the near-infrared spectroscopy (NIRS) combined with partial least squares regression (PLSR) to establish a quantitative analysis model of total nucleosides for online process monitoring of fermented Cordyceps powder preparation products. The model parameters indicate that the established model with good robustness and high measurement precision. It further clarifies that the model can be used for online process monitoring of fermented Cordyceps powder preparation products.

中文翻译：

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近红外光谱分析技术在冬虫夏草粉生产过程总核苷质量控制中的应用

产品质量控制是确保安全，有效性和稳定性的前提。但是，由于菌株种类和发酵过程的不同，质量存在明显差异。因此，应在临床使用中清楚地区分它们。其中，发酵过程对于获得稳定的产品质量至关重要。本研究旨在将近红外光谱分析技术引入发酵虫草粉的生产过程中，包括菌株培养，菌株传代，菌株发酵，菌株过滤，菌株干燥，菌株粉碎和菌株混合。首先，高效液相色谱（HPLC）用于测量30批次发酵虫草粉的生产过程中的总核苷含量，其中包括尿嘧啶，尿苷，腺嘌呤，用中药（TCM）指纹图谱分析鸟嘌呤，腺苷，过程稳定性和批间一致性，然后使用近红外光谱（NIRS）结合偏最小二乘回归（PLSR）建立总核苷定量分析模型用于发酵虫草粉制剂产品的在线过程监控。模型参数表明所建立的模型具有良好的鲁棒性和较高的测量精度。它进一步阐明该模型可用于发酵虫草粉制剂产品的在线过程监控。然后将近红外光谱（NIRS）与偏最小二乘回归（PLSR）结合，以建立总核苷的定量分析模型，以在线监测发酵的虫草粉制剂产品。模型参数表明所建立的模型具有良好的鲁棒性和较高的测量精度。它进一步阐明该模型可用于发酵虫草粉制剂产品的在线过程监控。然后将近红外光谱（NIRS）与偏最小二乘回归（PLSR）结合，以建立总核苷的定量分析模型，以在线监测发酵的虫草粉制剂产品。模型参数表明所建立的模型具有良好的鲁棒性和较高的测量精度。它进一步阐明该模型可用于发酵虫草粉制剂产品的在线过程监控。