Understanding the tissue distribution of phospholipids and glycerolipids in animal models enables promoting the pharmacokinetic study of drugs and related PK predictions. The measurement of lipid compositions in animal models, usually mice and rats, without a standardized approach hindered the accuracy of PBPK investigation. In this work, high resolution mass spectrometry was applied to profile the tissue distribution of phospholipids and glycerolipids in 12 organs/tissues of mice and rats. Using this method, not only the amounts of phospholipids and glycerolipids in each organ/tissue but also the fatty acid compositions were acquired. In order to explore the interspecies specificity of lipid distribution in different organs/tissues, three animal species including CD1 mice, NMRI mice, and Wister rats were used in this systematic study. Globally, more organ specificity was observed. It was found that the brain is the organ containing the most abundant phosphatidylserine lipids (PSs) in all three animal models, leading to brain tissues having the most concentrated acidic phospholipids. Diverse fatty acid compositions in each lipid class were clearly revealed. Certain tissues/organs also had a specific selection of unique fatty acid compositions, for example, unreferenced FA(18 : 2) in the brain. It turned out that the access of free fatty acids affects the incorporation of acyl chain in phospholipids and glycerolipids. In the analysis, ether lipids were also profiled with the observation of dominant ePEs in brain tissues. However, little interspecies difference was found for fatty acid constituents and tissues distribution of phospholipids and glycerolipids.

中文翻译：

---

CD1和NMRI小鼠和Wistar大鼠的全组织分布和膜脂质的脂肪酸组成的系统分析

了解动物模型中磷脂和甘油脂的组织分布可以促进药物的药代动力学研究和相关的PK预测。在没有标准化方法的情况下，在动物模型（通常为小鼠和大鼠）中脂质成分的测量阻碍了PBPK研究的准确性。在这项工作中，采用高分辨率质谱分析小鼠和大鼠的12个器官/组织中磷脂和甘油脂的组织分布。使用该方法，不仅获得了每个器官/组织中的磷脂和甘油脂的量，而且还获得了脂肪酸组合物。为了探索不同器官/组织中脂质分布的种间特异性，在该系统研究中使用了3种动物，包括CD1小鼠，NMRI小鼠和Wister大鼠。在全球范围内 观察到更多的器官特异性。发现在所有三种动物模型中，大脑都是含有最丰富的磷脂酰丝氨酸脂质（PSs）的器官，导致大脑组织具有最浓的酸性磷脂。清楚地揭示了每个脂质类别中的不同脂肪酸组成。某些组织/器官还具有特定脂肪酸组成的特定选择，例如大脑中未参考的FA（18：2）。结果表明，游离脂肪酸的进入会影响酰基链在磷脂和甘油脂中的掺入。在分析中，还通过观察脑组织中占主导地位的ePEs对乙醚脂质进行了分析。然而，几乎没有种间差异的脂肪酸成分和磷脂和甘油脂的组织分布。