This study aimed to validate an analytical method to determine DNA concentration using standard reference material (NIST SRM 2372) and Sprague Dawley rat and human DNA. Microvolumes were used to analyse DNA samples. Linearity showed correlation coefficients higher than R ≥ 0.9950, and the precision value was ≤2% CV. Trueness based on bias and the percentage of recovery showed bias values lower than Z-test with a 95% confidence level and a recovery percentage within the range (% Rec = 100% ± 5%), and the stability of the samples was 60 days (2–4°C).

中文翻译：

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通过 NanoDrop 分光光度计进行 DNA 定量：使用标准参考材料和 Sprague Dawley 大鼠和人类 DNA 进行方法学验证

本研究旨在验证使用标准参考材料 (NIST SRM 2372) 以及 Sprague Dawley 大鼠和人类 DNA 确定 DNA 浓度的分析方法。微体积用于分析 DNA 样本。线性相关系数R≥0.9950  ，精密度≤2%CV。基于偏差和回收百分比的真实性显示偏差值低于Z检验，置信度为 95%，回收百分比在范围内 (% Rec = 100% ± 5%)，并且样品的稳定性为 60 天(2–4°C)。