There is currently no specific prophylaxis or vaccine against Crimean-Congo haemorrhagic fever virus (CCHFV). Crimean-Congo haemorrhagic fever (CCHF) is a severe febrile illness transmitted by Hyalomma ticks in endemic areas, handling of infected livestock or care of infected patients. We report here the successful protection against CCHFV-mediated disease in a non-human primate disease model. Cynomolgus macaques were vaccinated with a DNA-based vaccine using in vivo electroporation-assisted delivery. The vaccine contained two plasmids encoding the glycoprotein precursor (GPC) and the nucleoprotein (NP) of CCHFV. Animals received three vaccinations and we recorded potent antibody and T cell responses after vaccination. While all sham-vaccinated animals developed viraemia, high tissue viral loads and CCHF-induced disease, the NP + GPC vaccinated animals were significantly protected. In conclusion, this is evidence of a vaccine that can protect against CCHFV-induced disease in a non-human primate model. This supports clinical development of the vaccine to protect groups at risk for contracting the infection.

目前没有针对克里米亚-刚果出血热病毒 (CCHFV) 的特异性预防措施或疫苗。克里米亚-刚果出血热 (CCHF) 是一种由Hyalomma传播的严重发热性疾病流行地区的蜱虫、感染牲畜的处理或感染患者的护理。我们在此报告在非人类灵长类动物疾病模型中成功预防 CCHFV 介导的疾病。使用体内电穿孔辅助递送，用基于 DNA 的疫苗对食蟹猴进行了疫苗接种。该疫苗含有两个编码CCHFV的糖蛋白前体（GPC）和核蛋白（NP）的质粒。动物接受了三种疫苗接种，我们在疫苗接种后记录了有效的抗体和 T 细胞反应。虽然所有假疫苗接种的动物都出现了病毒血症、高组织病毒载量和 CCHF 诱导的疾病，但 NP + GPC 接种的动物受到了显着保护。总之，这是一种疫苗可以在非人类灵长类动物模型中预防 CCHFV 诱发疾病的证据。