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Smart Adhesive Peptide Nanofibers for Cell Capture and Release
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2020-11-30 , DOI: 10.1021/acsbiomaterials.0c01485 Tiankuo Wang 1 , Yiran Li 1 , Juan Wang 1 , Ying Xu 1 , Yifang Chen 2, 3 , Zilin Lu 2, 3 , Wei Wang 1 , Bin Xue 1 , Ying Li 2, 3 , Yi Cao 1
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2020-11-30 , DOI: 10.1021/acsbiomaterials.0c01485 Tiankuo Wang 1 , Yiran Li 1 , Juan Wang 1 , Ying Xu 1 , Yifang Chen 2, 3 , Zilin Lu 2, 3 , Wei Wang 1 , Bin Xue 1 , Ying Li 2, 3 , Yi Cao 1
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Efficient cell capture and release methods are important for single-cell analysis of pathological samples. It requires not only strong cell binding but also mild cell release to maximize the number of collected cells while maintaining their viability. Here, we report a smart cell capture and release system based on self-assembling adhesive peptide nanofibers. We installed a versatile surface binding motif, 3, 4-dihydroxyphenylalanine to the C-terminus of a self-assembling peptide. We show that the designed peptide can self-assemble at physiological pH to establish strong cell and substrate binding. The binding strength is dramatically reduced upon the dissembling of the peptide fibers triggered by raising the pH to slightly basic. We demonstrate the efficient capture of four different cells using this system. The capture rates are comparable to fibrin glue and the released cells retain higher viability than those released by enzymatic digestion approaches. Given that this method is highly efficient, biocompatible, and easy to implement, we anticipate that this approach can be widely applied to cell capture and release for single cell analysis and cell therapy.
中文翻译：
用于细胞捕获和释放的智能粘附肽纳米纤维
高效的细胞捕获和释放方法对于病理样品的单细胞分析非常重要。它不仅需要强大的细胞结合力，而且还需要温和的细胞释放以最大化收集的细胞数量，同时保持其活力。在这里，我们报告了一种基于自组装粘性肽纳米纤维的智能细胞捕获和释放系统。我们在自组装肽的C端安装了通用的表面结合基序3，4-二羟基苯丙氨酸。我们表明，设计的肽可以在生理pH下自组装，建立牢固的细胞和底物结合。通过将pH升高至略碱性触发肽纤维的分解，结合强度会大大降低。我们展示了使用该系统对四个不同细胞的有效捕获。捕获率可与纤维蛋白胶媲美，并且所释放的细胞比酶消化方法所释放的细胞保留更高的生存力。鉴于此方法高效，生物相容且易于实施，我们预计该方法可广泛应用于单细胞分析和细胞治疗的细胞捕获和释放。
更新日期：2020-12-14
中文翻译：
用于细胞捕获和释放的智能粘附肽纳米纤维
高效的细胞捕获和释放方法对于病理样品的单细胞分析非常重要。它不仅需要强大的细胞结合力，而且还需要温和的细胞释放以最大化收集的细胞数量，同时保持其活力。在这里，我们报告了一种基于自组装粘性肽纳米纤维的智能细胞捕获和释放系统。我们在自组装肽的C端安装了通用的表面结合基序3，4-二羟基苯丙氨酸。我们表明，设计的肽可以在生理pH下自组装，建立牢固的细胞和底物结合。通过将pH升高至略碱性触发肽纤维的分解，结合强度会大大降低。我们展示了使用该系统对四个不同细胞的有效捕获。捕获率可与纤维蛋白胶媲美，并且所释放的细胞比酶消化方法所释放的细胞保留更高的生存力。鉴于此方法高效，生物相容且易于实施，我们预计该方法可广泛应用于单细胞分析和细胞治疗的细胞捕获和释放。
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