Abstract

To broaden the knowledge regarding regenerative medicine in natural rubber latex (NRL) nanoparticles, we have examined the chondrogenesis of human mesenchymal stem cells (hMSCs) with NRL nanoparticles under hypoxia condition. We have successfully fabricated the cartilage/NRL biocomposites via hMSCs spheroid under hypoxic condition, where the administration of NRL nanoparticles exhibits the suppression of the spheroid contraction due to the cellular proliferation of hMSCs. It has been found that the NRL nanoparticles acted as a main component, which provides surface heterogeneity of the spheroid, leading to the mechanically stable structure with higher modulus in comparison with the control as revealed by atomic force microscopy microindentation. Under hypoxia condition, the effective gene expression of in vitro chondrogenesis of hMSCs with administration of NRL was confirmed as revealed by the chondrogenic gene expression analysis compared with that under normoxia condition.

摘要

为了拓宽有关天然橡胶胶乳（NRL）纳米颗粒中再生医学的知识，我们检查了缺氧条件下带有NRL纳米颗粒的人间充质干细胞（hMSCs）的软骨形成。我们已经成功地在缺氧条件下通过hMSCs球状体制备了软骨/ NRL生物复合材料，其中由于hMSCs的细胞增殖，NRL纳米颗粒的给药显示出对球状体收缩的抑制作用。已经发现，NRL纳米颗粒充当主要成分，提供了球体的表面异质性，与原子力显微镜显微压印所显示的对照相比，导致了具有更高模量的机械稳定结构。缺氧条件下体外有效基因表达 与正常氧条件下相比，通过软骨基因表达分析可知，应用了NRL的hMSCs发生了软骨形成。