This study aimed to investigate the therapeutic effect of pulsed electromagnetic field (PEMF) on bone wound in rats as a potential therapy for bone fracture-related conditions. Male rats, aged 3 months, were used to construct model of bone wounding. Wound models were randomly selected to receive PEMF therapy at 1 to 10 mT intensity. Models that did not receive PEMF therapy were used as control. The serum concentrations of calcium (Ca), phosphorus (P) and alkaline phosphatase (ALP) were determined. Bone density and biomechanical properties of callus were measured using a tensile tester. Compared with control, rats subjected to PEMF therapy had similar weight gain, but significantly higher levels of serum Ca and ALP (P < .05) at 5 and 10 mT, while the serum level of P remained unchanged after PEMF therapy. The bone mineral density of callus increased after the therapy, particularly, after 5 and 10 mT therapy (P < .05). Biomechanical measurements showed that 21 days after the therapy, the maximum load, fracture load, elastic load and bending energy were significantly greater in rats receiving 5 and 10 mT PEMF therapy as compared with control (P < .05). Our experiments demonstrate that PEMF at 5 and 10 mT can significantly accelerate wound healing and enhance the repairing ability of bone tissue.

中文翻译：

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脉冲电磁场​​对大鼠骨创面愈合的治疗作用


本研究旨在探讨脉冲电磁场​​ (PEMF) 对大鼠骨伤口的治疗效果，作为骨折相关疾病的潜在疗法。采用3月龄雄性大鼠构建骨损伤模型。随机选择伤口模型接受 1 至 10 mT 强度的 PEMF 治疗。未接受 PEMF 治疗的模型用作对照。测定钙（Ca）、磷（P）和碱性磷酸酶（ALP）的血清浓度。使用拉伸测试仪测量愈伤组织的骨密度和生物力学特性。与对照组相比，接受PEMF治疗的大鼠体重增加相似，但在5和10 mT时血清Ca和ALP水平显着升高（P< .05），而PEMF治疗后血清P水平保持不变。治疗后，尤其是 5 和 10 mT 治疗后，愈伤组织的骨矿物质密度有所增加 (P < .05)。生物力学测量显示，治疗后 21 天，接受 5 和 10 mT PEMF 治疗的大鼠的最大载荷、断裂载荷、弹性载荷和弯曲能显着高于对照组 (P < .05)。我们的实验表明，5 和 10 mT 的 PEMF 可以显着加速伤口愈合并增强骨组织的修复能力。