Abstract

Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae), populations exhibit varying levels of resistance to several insecticide classes, including pyrethroids. Artificial laboratory selection was conducted to investigate mechanisms underlying fenpropathrin resistance. After ten generations of selection, the LC₅₀ for fenpropathrin increased from 0.18 to 11.60 ng/μL, and the associated resistance ratio increased by 96.67-fold. The realized heritability of resistance (h²) to fenpropathrin changed from 0.17 to 0.44 during the first and second round of selection, respectively. We did not find high cross-resistance to imidacloprid (RR = 3.58) or dimethoate (RR = 1.77) in the fenpropathrin-selected strain, but found cross-resistance to bifenthrin (RR = 28.21). RT-qPCR analysis showed that expression of CYP6A2-1 was significantly increased in the selected population relative to the laboratory susceptible population. Our data indicate that D. citri has the capacity to develop a high level of fenpropathrin resistance following persistent selection and that cytochrome P450 (CYP) and glutathione S-transferase (GST) metabolic detoxification may be contributing mechanisms. Rotation programs that include neonicotinoids and organophosphates should manage resistance to fenpropathrin for D. citri.

摘要

亚洲柑橘木虱Diaphorina citri Kuwayama（半翅目：Liviidae）种群对多种杀虫剂（包括拟除虫菊酯）表现出不同程度的抗性。进行人工实验室选择以研究甲氰菊酯抗性的潜在机制。经过十代选择，甲氰菊酯的LC ₅₀从0.18增加到11.60 ng/μL，相关耐药比增加了96.67倍。已实现的抗性遗传力 ( h ²) 在第一轮和第二轮选择期间分别从 0.17 变为 0.44。在甲氰菊酯选择的菌株中，我们没有发现对吡虫啉 (RR = 3.58) 或乐果 (RR = 1.77) 的高交叉抗性，但发现对联苯菊酯 (RR = 28.21) 的交叉抗性。RT-qPCR 分析表明，相对于实验室易感人群，所选人群中CYP6A2-1的表达显着增加。我们的数据表明D. citri有能力在持续选择后产生高水平的甲氰菊酯抗性，细胞色素 P450 (CYP) 和谷胱甘肽 S-转移酶 (GST) 代谢解毒可能是促成机制。包括新烟碱类和有机磷酸盐在内的轮换计划应该管理D. citri对甲氰菊酯的抗性。