Abstract

Pachira aquatica is a species used for medicinal and food purposes and has numerous phytochemicals that may have systemic toxic effects and damage to genetic material. This study aimed to evaluate acute and short-term oral toxicity, as well as genotoxic and clastogenic effects of oil extracted from P. aquatica (PASO) seeds in rats and Drosophila melanogaster. The results obtained with biochemical and hematological analyses did not show significant changes in any evaluated parameters when compared with reference values for the species used in the study. Data from the histopathological analysis corroborated results found in this study. These findings indicate low acute and short-term toxicity following oral PASO exposure in rats under the experimental conditions tested. Tests performed in rats showed that PASO did not present significant genotoxic or clastogenic effects on the cells analyzed with the three doses tested. Treatment with PASO in the offspring of HB crossing, which showed high cytochrome P450 levels, did not exhibit genotoxic activity, as demonstrated by the SMART test. These results suggest that products from the hepatic oil metabolism did not show genotoxicity under the conditions tested. Together, the results indicate that, under the experimental conditions tested, PASO is safe for repeated intake. As PASO exhibited low potential to cause harmful effects on living organisms, our study encourages further research aimed at assessing its pharmacological activity, since it is a widely consumed plant.

摘要

Pachira wateraqua是一种用于药用和食品目的的物种，含有许多可能对遗传物质产生全身毒性作用和损害的植物化学物质。本研究旨在评估大鼠和黑腹果蝇种子中提取的油的急性和短期口服毒性，以及遗传毒性和致裂作用。. 与研究中使用的物种的参考值相比，通过生化和血液学分析获得的结果未显示任何评估参数的显着变化。来自组织病理学分析的数据证实了本研究中发现的结果。这些发现表明，在测试的实验条件下，大鼠经口 PASO 暴露后的急性和短期毒性较低。在大鼠中进行的测试表明，PASO 对用三种测试剂量分析的细胞没有显着的基因毒性或致畸作用。用 PASO 治疗 HB 杂交的后代，显示出高细胞色素 P450 水平，没有表现出遗传毒性活性，如 SMART 测试所示。这些结果表明，来自肝油代谢的产物在测试条件下没有表现出遗传毒性。总之，结果表明，在所测试的实验条件下，PASO 对于重复摄入是安全的。由于 PASO 对生物体造成有害影响的可能性较低，因此我们的研究鼓励进一步研究旨在评估其药理活性，因为它是一种广泛食用的植物。