Abstract

Because of diverse adverse effects of carbendazim (CBZ) upon the human body and environment, a simple method for the determination of this molecule is urgently needed. Therefore, a method based on an aptamer was developed. An aptamer of carbendazim, CZ12, labeled with a fluorescent molecule (FAM), was adsorbed on graphene oxide (GO) by π-π stacking. The fluorescence resonance energy transfer (FRET) between the molecule and GO was used to rapidly quench the fluorescence signal. In the presence of carbendazim, the fluorescent molecule selectively combines with carbendazim, thereby greatly reducing the binding efficiency of the former with GO. Thus, by mixing a FAM-labeled carbendazim aptamer, GO, and food samples, the carbendazim content was determined from the fluorescence measurements. The limit of quantification was 10.0 ng/mL for strawberry, banana, pitaya, and Panax notoginseng samples. The linear range was from 0 to 125 ng/mL with a linear correlation coefficient equal to 0.9918.

摘要

由于多菌灵 (CBZ) 对人体和环境的多种不利影响，迫切需要一种简单的方法来测定该分子。因此，开发了一种基于适体的方法。用荧光分子 (FAM) 标记的多菌灵适体 CZ12 通过 π-π 堆积吸附在氧化石墨烯 (GO) 上。分子与 GO 之间的荧光共振能量转移 (FRET) 用于快速淬灭荧光信号。在多菌灵存在下，荧光分子选择性地与多菌灵结合，从而大大降低了前者与GO的结合效率。因此，通过混合 FAM 标记的多菌灵适体、GO 和食品样品，可通过荧光测量确定多菌灵含量。草莓的定量限为 10.0 ng/mL，三七样品。线性范围为 0 到 125 ng/mL，线性相关系数等于 0.9918。