Background Platelet-rich plasma (PRP), which represents a valuable source of growth factors, is increasingly being applied in regenerative medicine. Recent findings suggest the feasibility of using PRP in the treatment of infertility secondary to refractory thin endometrium. Mesenchymal stem/stromal cells (MSCs) of the endometrium are an essential cellular component responsible for extracellular matrix remodeling, angiogenesis, cell-to-cell communication, and postmenstrual tissue repair. Using a rat model, we examine the effects of autologous PRP on MSCs isolated from the uterus and compare them with the effects of autologous ordinary plasma (OP) and complete growth medium. Methods MSCs were isolated from uterine tissues via enzymatic disaggregation. Flow cytometry immunophenotyping of the primary cell cultures was complemented by immunocytochemistry for Ki-67 and vimentin. The ability of MSCs to differentiate in osteo-, chondro-, and adipogenic directions was assessed using differentiation-inducing media. The levels of autophagy and apoptosis markers, as well as the levels of matrix metalloproteinase 9 (MMP9) and estrogen receptor α, were assessed by western blotting. Results After 24 h incubation, the proliferation index of the PRP-treated MSC cultures was significantly higher than that of the MSC cultures treated with complete growth medium. PRP treatment elevated production of LC3B protein, an autophagy marker, while OP treatment upregulated the expression of stress-induced protein p53 and extracellular enzyme MMP9. The results indicate practical relevance and validity for PRP use in the treatment of infertility.

中文翻译：

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富血小板血浆对大鼠子宫间充质干细胞的影响

背景富血小板血浆（PRP）是一种有价值的生长因子来源，越来越多地应用于再生医学。最近的研究结果表明，使用 PRP 治疗继发于难治性薄子宫内膜的不孕症是可行的。子宫内膜的间充质干/基质细胞 (MSCs) 是负责细胞外基质重塑、血管生成、细胞间通讯和经后组织修复的重要细胞成分。使用大鼠模型，我们检查了自体 PRP 对从子宫分离的 MSCs 的影响，并将其与自体普通血浆 (OP) 和完全生长培养基的影响进行比较。方法通过酶解从子宫组织中分离MSCs。Ki-67 和波形蛋白的免疫细胞化学补充了原代细胞培养物的流式细胞仪免疫表型分析。使用分化诱导培养基评估 MSCs 在骨、软骨和脂肪形成方向上的分化能力。通过蛋白质印迹评估自噬和凋亡标志物的水平，以及基质金属蛋白酶 9 (MMP9) 和雌激素受体 α 的水平。结果培养24 h后，经PRP处理的MSC培养物的增殖指数显着高于经完全培养基处理的MSC培养物。PRP 处理提高了 LC3B 蛋白（一种自噬标志物）的产生，而 OP 处理上调了应激诱导蛋白 p53 和细胞外酶 MMP9 的表达。