Direct Antimicrobial Susceptibility Testing on Clinical Urine Samples by Optical Tracking of Single Cell Division Events,Small

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Direct Antimicrobial Susceptibility Testing on Clinical Urine Samples by Optical Tracking of Single Cell Division Events
Small ( IF 13.0 ) Pub Date : 2020-11-30 , DOI: 10.1002/smll.202004148
Fenni Zhang ₁ , Jiapei Jiang _{1,

2} , Michelle McBride ₁ , Yunze Yang ₁ , Manni Mo _{1,

3} , Rafael Iriya _{1,

4} , Joseph Peterman ₁ , Wenwen Jing ₁ , Thomas Grys ₅ , Shelley E Haydel _{1,

6} , Nongjian Tao _{1,

4} , Shaopeng Wang ₁

Affiliation

With the increasing prevalence of antibiotic resistance, the need to develop antimicrobial susceptibility testing (AST) technologies is urgent. The current challenge has been to perform the antibiotic susceptibility testing in short time, directly with clinical samples, and with antibiotics over a broad dynamic range of clinically relevant concentrations. Here, a technology for point‐of‐care diagnosis of antimicrobial‐resistant bacteria in urinary tract infections, by imaging the clinical urine samples directly with an innovative large volume solution scattering imaging (LVSi) system and analyzing the image sequences with a single‐cell division tracking method is developed. The high sensitivity of single‐cell division tracking associated with large volume imaging enables rapid antibiotic susceptibility testing directly on the clinical urine samples. The results demonstrate direct detection of bacterial infections in 60 clinical urine samples with a 60 min LVSi video, and digital AST of 30 positive clinical samples with 100% categorical agreement with both the clinical culture results and the on‐site agar plating validation results. This technology provides opportunities for precise antibiotic prescription and proper treatment of the patient within a single clinic visit.

中文翻译：

通过单细胞分裂事件的光学跟踪对临床尿液样本进行直接抗菌药物敏感性测试

随着抗生素耐药性的日益流行，迫切需要开发抗菌药敏试验 (AST) 技术。当前的挑战是在短时间内直接对临床样本进行抗生素敏感性测试，并在临床相关浓度的广泛动态范围内进行抗生素敏感性测试。在这里，通过创新的大体积溶液散射成像 (LVSi) 系统直接对临床尿液样本进行成像，并使用单细胞分析图像序列，对尿路感染中的抗菌素耐药菌进行即时诊断的技术开发了分区跟踪方法。与大体积成像相关的单细胞分裂跟踪的高灵敏度能够直接对临床尿样进行快速的抗生素药敏试验。结果表明，使用 60 分钟 LVSi 视频直接检测 60 个临床尿液样本中的细菌感染，以及 30 个阳性临床样本的数字 AST，与临床培养结果和现场琼脂平板验证结果 100% 绝对一致。该技术提供了在单次门诊就诊时精确处方抗生素和对患者进行适当治疗的机会。30 个阳性临床样本的数字 AST 与临床培养结果和现场琼脂平板验证结果 100% 绝对一致。该技术提供了在单次门诊就诊时精确处方抗生素和对患者进行适当治疗的机会。30 个阳性临床样本的数字 AST 与临床培养结果和现场琼脂平板验证结果 100% 绝对一致。该技术提供了在单次门诊就诊时精确处方抗生素和对患者进行适当治疗的机会。

更新日期：2020-12-28

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