DREB transcription factors regulate the expression of stress‐responsive genes, and thus play an important role in plant stress response to abiotic stresses. To obtain a global expression profile of soybean DREB genes under drought stress, we first explored the soybean genome to identify DREB family genes, and then analysed a set of transcriptome data of drought stress to verify their involvement in stress response. We identified 73 DREB family genes from the soybean genome. These DREB genes were further divided into six subgroups basing on the phylogenetic analysis. Gene structure analysis showed that most DREB genes have a single exon without intron. Soybean DREB genes were unevenly distributed on 19 chromosomes. We further identified 186 putative target genes of soybean DREB proteins and found that these targeted genes were significantly enriched in metabolism pathways of fructose and mannose. The analysis of transcriptome data showed that DREB genes were differentially expressed during different periods of drought, indicating that the DREB family genes were responsive to drought stress.

中文翻译：

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大豆DREB基因家族的全基因组分析：针对干旱胁迫的鉴定，基因组组织和表达谱

DREB转录因子调节胁迫响应基因的表达，因此在植物对非生物胁迫的胁迫响应中起重要作用。为了获得干旱胁迫下大豆DREB基因的全球表达谱，我们首先探索了大豆基因组以鉴定DREB家族基因，然后分析了一组干旱胁迫的转录组数据以验证其参与胁迫响应。我们从大豆基因组中鉴定出73个DREB家族基因。根据系统发育分析，将这些DREB基因进一步分为六个亚组。基因结构分析表明，大多数DREB基因只有一个外显子，没有内含子。大豆DREB基因在19条染色体上分布不均。我们进一步鉴定了186个大豆DREB蛋白的推定靶基因，发现这些靶基因在果糖和甘露糖的代谢途径中显着丰富。转录组数据分析表明，DREB基因在干旱的不同时期表达差异，表明DREB家族基因对干旱胁迫有反应。