Our aim was to develop a novel medium for the large‐scale production of protein‐rich Arthrospira with potential applications as a biofertilizer. The novel culture medium, termed as FM‐II, was formulated using low‐cost commercial chemicals and specifically designed to improve protein production. Both Arthrospira platensis and Arthrospira maxima were produced using FM‐II and Arnon medium, which was used as a control. Photosynthetic status of the cells, which was checked by measuring chlorophyll fluorescence, biomass dry weight and protein content, was assessed daily. Arthrospira platensis had higher biomass and protein productivities than A. maxima when cultured in both control and FM‐II media. Incorporation of varied micronutrients into FM‐II formulation did not improve biomass productivity. Maximum biomass dry weight in FM‐II and control medium was 2.9 and 2.5 g · L⁻¹, respectively. Total protein content of the biomass ranged between 55% and 65%, suggesting potential for being used in the development of high‐value agricultural products. As some nutrients were discarded unused, the initial content of phosphates and bicarbonates was reduced by 75% and 50%, respectively, without affecting the process productivity. Results reported herein could promote the production and utilization of Arthrospira platensis by significantly reducing productions costs and therefore increasing the feasibility of the process.

中文翻译：

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大规模生产富含蛋白质的关节螺旋藻的新培养基的优化（振荡菌，蓝藻）

我们的目标是开发一种新型培养基，用于大规模生产富含蛋白质的节肢动物，并将其潜在地用作生物肥料。这种新型培养基称为FM-II，是使用低成本的商业化学品配制的，专门用于提高蛋白质产量。两个节旋藻和节旋藻最大值用FM-II和亚嫩介质，将其用作对照制备。每天评估细胞的光合作用状态，该状态通过测量叶绿素荧光，生物量干重和蛋白质含量来检查。节肢动物的生物量和蛋白质生产力均比极大曲霉高。同时在对照和FM-II培养基中培养时。在FM-II配方中加入多种微量营养素并不能提高生物量的生产率。FM-II和对照培养基中的最大生物量干重分别为2.9和2.5 g·L ^-1。生物质的总蛋白质含量介于55％至65％之间，这表明其可用于开发高价值农产品。由于一些养分被弃置而未使用，磷酸盐和碳酸氢盐的初始含量分别降低了75％和50％，而不会影响过程生产率。本文报道的结果可通过显着降低生产成本并因此增加该方法的可行性来促进白色节肢动物的生产和利用。