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Anti‐inflammatory and anticancer activities of Naringenin‐loaded liquid crystalline nanoparticles in vitro
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2020-11-29 , DOI: 10.1111/jfbc.13572
Ridhima Wadhwa 1, 2 , Keshav Raj Paudel 2, 3 , Li Hian Chin 4 , Chian Ming Hon 4 , Thiagarajan Madheswaran 5 , Gaurav Gupta 6 , Jithendra Panneerselvam 5 , Thangavelu Lakshmi 7 , Sachin Kumar Singh 8 , Monica Gulati 8 , Harish Dureja 9 , Alan Hsu 10 , Meenu Mehta 1, 2 , Krishnan Anand 11 , Hari Prasad Devkota 12 , Jestin Chellian 13 , Dinesh Kumar Chellappan 13 , Philip M Hansbro 2, 3, 10 , Kamal Dua 1, 2, 10
Affiliation  

In this study, we had developed Naringenin‐loaded liquid crystalline nanoparticles (LCNs) and investigated the anti‐inflammatory and anticancer activities of Naringenin‐LCNs against human airway epithelium‐derived basal cells (BCi‐NS1.1) and human lung epithelial carcinoma (A549) cell lines, respectively. The anti‐inflammatory potential of Naringenin‐LCNs evaluated by qPCR revealed a decreased expression of IL‐6, IL‐8, IL‐1β, and TNF‐α in lipopolysaccharide‐induced BCi‐NS1.1 cells. The activity of LCNs was comparable to the positive control drug Fluticasone propionate (10 nM). The anticancer activity was studied by evaluating the antiproliferative (MTT and trypan blue assays), antimigratory (scratch wound healing assay, modified Boyden chamber assay, and immunoblot), and anticolony formation activity in A549 cells. Naringenin LCNs showed promising antiproliferative, antimigratory, and anticolony formation activities in A549 cells, in vitro. Therefore, based on our observations and results, we conclude that Naringenin‐LCNs may be employed as a potential therapy‐based intervention to ameliorate airway inflammation and to inhibit the progression of lung cancer.

中文翻译:

装有柚皮素的液晶纳米粒子的体外抗炎和抗癌活性

在这项研究中,我们开发了装有Naringenin的液晶纳米颗粒(LCN),并研究了Naringenin-LCNs对人气道上皮来源的基底细胞(BCi-NS1.1)和人肺上皮癌( A549)细胞系。qPCR评估的柚皮素LCNs的抗炎潜力显示,脂多糖诱导的BCi-NS1.1细胞中IL-6,IL-8,IL-1β和TNF-α的表达降低。LCN的活性与阳性对照药丙酸氟替卡松(10 nM)相当。通过评估A549细胞中的抗增殖活性(MTT和锥虫蓝试验),抗迁移性(划痕伤口愈合试验,改良的Boyden小室试验和免疫印迹)和抗菌落形成活性,研究了抗癌活性。柚皮素LCNs在A549细胞中显示出有希望的抗增殖,抗迁移和抗菌落形成活性。因此,根据我们的观察和结果,我们得出结论,柚皮素-LCNs可以作为一种潜在的基于治疗的干预措施,以改善气道炎症并抑制肺癌的进展。
更新日期:2021-01-19
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