Chitin was isolated from Pacific white shrimp (Litopenaeus vannamei) shell by demineralization and deproteinization using 1 M HCl (1:20, w/v) for 2 hr and 1 M NaOH (1:30 w/v) for 80 min at 70ºC, respectively, with 29.96% optimum yield. Thereafter, the chitin was deacetylated at various temperatures for different times, in which the chitosan prepared at 130ºC for 4 hr (CS‐130‐4) showed higher yield (73.11%), crystallinity index (19.75%), and 85.28% degree of deacetylation (DDA) as measured by ¹H‐NMR. CS‐130‐4 was then conjugated to epigallocatechin gallate (EGCG) at various concentrations (2–8%, w/w of chitosan). CS‐130‐4 was grafted with 8% EGCG (CE‐8) had the higher conjugation efficiency (92.63%) and antimicrobial/antioxidant activities as compared to other conjugates (p < .05). ¹H‐NMR analysis also confirmed the successful conjugation of CE‐8. All the conjugates were completely water soluble. Therefore, CE‐8 may be used as the natural antimicrobial and antioxidant agents in various food products.

中文翻译：

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太平洋白虾（Litopenaeus vannamei）壳壳聚糖及其与没食子儿茶素没食子酸酯的结合物：抗氧化和抗菌活性

几丁质是从太平洋白虾（分离的凡纳滨对虾）由去矿化和脱蛋白质使用1M的HCl壳（1:20，W / V）2小时和1M NaOH（1:30重量/体积）在70℃80分钟，分别具有29.96％的最佳收率。之后，几丁质在不同温度下脱乙酰的时间不同，其中在130ºC下制备4小时的壳聚糖（CS‐130‐4）表现出更高的收率（73.11％），结晶度指标（19.75％）和85.28％的壳聚糖度。脱乙酰化（DDA），通过¹ H-NMR测量。然后将CS‐130‐4以各种浓度（2-8％，w / w壳聚糖）与表没食子儿茶素没食子酸酯（EGCG）偶联。与其他缀合物相比，CS‐130‐4嫁接了8％EGCG（CE‐8）具有更高的缀合效率（92.63％）和抗微生物/抗氧化活性（p  <.05）。¹ H-NMR分析也证实了CE-8的成功偶联。所有结合物都是完全水溶性的。因此，CE-8可用作各种食品中的天然抗菌剂和抗氧化剂。