The design of novel wound dressings for chronic wound treatment is still of great importance. One of the promising approaches is application of mesenchymal stem cells (MSCs), immobilized on a flexible polymer film, for healing. In this study, blended films based on polyvinyl alcohol (PVA) and pectin with different component ratio have been prepared by solution casting method and evaluated. Physicochemical properties of the formed PVA/pectin films, including their morphology, wettability, swelling, stability, mechanical characteristics, have been studied. We demonstrated that the surface of PVA/pectin films could be modified by ultraviolet or dielectric barrier discharge plasma exposure. After both ultraviolet and plasma treatment, the hydrophilicity of PVA/pectin films increased. It has been shown that additional crosslinking of PVA/pectin films with glutaraldehyde resulted in reinforcement of their structure. MSCs were cultured on neat and modified PVA/pectin samples to evaluate the effects of film characteristics and composition on cell behavior. It has been determined that MSCs effectively adhered to glutaraldehyde-crosslinked PVA/pectin films and formed on them the monolayer culture of fibroblast-like cells. The additional modification of PVA/pectin films with collagen resulted in enhancement of MSCs adhesion. Our results show that the obtained PVA/pectin films with adhered MSCs can be suggested for potential application as a part of novel complex wound dressings.

中文翻译：

---

聚乙烯醇和果胶混合膜：制备、表征和间充质干细胞附着


设计用于慢性伤口治疗的新型伤口敷料仍然非常重要。其中一种有前景的方法是应用固定在柔性聚合物薄膜上的间充质干细胞（MSC）进行治疗。本研究采用溶液流延法制备了不同成分比例的聚乙烯醇（PVA）和果胶的共混薄膜并进行了评价。研究了所形成的PVA/果胶薄膜的物理化学性质，包括其形态、润湿性、溶胀性、稳定性、机械特性。我们证明，PVA/果胶薄膜的表面可以通过紫外线或介电阻挡放电等离子体暴露进行改性。经过紫外线和等离子体处理后，PVA/果胶薄膜的亲水性增加。研究表明，PVA/果胶薄膜与戊二醛的额外交联可增强其结构。在纯的和改性的 PVA/果胶样品上培养 MSC，以评估薄膜特性和成分对细胞行为的影响。已确定MSCs有效粘附于戊二醛交联的PVA/果胶膜上并在其上形成成纤维细胞样细胞的单层培养物。用胶原蛋白对 PVA/果胶膜进行额外修饰可增强 MSC 粘附。我们的结果表明，所获得的带有粘附 MSC 的 PVA/果胶薄膜可作为新型复杂伤口敷料的一部分进行潜在应用。