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Human decellularized adipose matrix derived hydrogel assists mesenchymal stem cells delivery and accelerates chronic wound healing
Journal of Biomedical Materials Research Part A ( IF 3.9 ) Pub Date : 2020-11-30 , DOI: 10.1002/jbm.a.37133
Zhaoyang Chen 1, 2 , Bowen Zhang 3, 4 , Jun Shu 2 , Haiyang Wang 3, 5 , Yudi Han 2 , Quan Zeng 3, 5 , Youbai Chen 2 , Jiafei Xi 3, 5 , Ran Tao 2 , Xuetao Pei 3, 5 , Wen Yue 3, 5 , Yan Han 2
Affiliation  

Biological scaffolds based stem cell delivery methods have emerged as a promising approach for tissue repair and regeneration. Here we developed a hydrogel biological scaffold from human decellularized adipose matrix (hDAM) for human adipose-derived stem cells (hASCs) delivery to accelerate chronic wound healing. The hDAM hydrogel was prepared by pepsin mediated digestion and pH controlled neutralization. The morphology, survival, proliferation, and angiogenic paracrine activity of hASCs cultured in the hydrogel were assessed. Moreover, the therapeutic efficacy of the hASCs-hydrogel composite for impaired wound healing was evaluated by using a full-thickness wound model on diabetic mouse. The developed hDAM hydrogel was a thermosensitive hydrogel, presented the biochemical complexity of native extracellular matrix and formed a porous nanofiber structure after gelation. The hydrogel can support hASCs adhesion, survival, and proliferation. Compared to standard culture condition, hASCs cultured in the hydrogel exhibited enhanced paracrine activity with increased secretion of hepatocyte growth factor. In the diabetic mice model with excisional full-thickness skin wounds, mice treated with the hASCs-hydrogel composite displayed accelerated wound closure and increased neovascularization. Our results suggested that the developed hDAM hydrogel can provide a favorable microenvironment for hASCs with augmented regeneration potential to accelerate chronic wound healing.

中文翻译:

人类脱细胞脂肪基质衍生的水凝胶有助于间充质干细胞输送并加速慢性伤口愈合

基于生物支架的干细胞递送方法已成为一种有前途的组织修复和再生方法。在这里,我们从人类脱细胞脂肪基质 (hDAM) 开发了一种水凝胶生物支架,用于人类脂肪干细胞 (hASC) 递送,以加速慢性伤口愈合。通过胃蛋白酶介导的消化和 pH 控制的中和制备 hDAM 水凝胶。评估了在水凝胶中培养的 hASCs 的形态、存活、增殖和血管生成旁分泌活性。此外,通过在糖尿病小鼠身上使用全层伤口模型,评估了 hASCs-水凝胶复合材料对伤口愈合受损的治疗效果。开发的hDAM水凝胶是一种热敏水凝胶,提出了天然细胞外基质的生化复杂性,并在凝胶化后形成多孔纳米纤维结构。水凝胶可以支持 hASCs 的粘附、存活和增殖。与标准培养条件相比,在水凝胶中培养的 hASCs 表现出增强的旁分泌活性,同时肝细胞生长因子的分泌增加。在切除全层皮肤伤口的糖尿病小鼠模型中,用 hASCs-水凝胶复合物治疗的小鼠表现出加速的伤口闭合和增加的新生血管形成。我们的研究结果表明,开发的 hDAM 水凝胶可以为 hASCs 提供有利的微环境,增强再生潜力以加速慢性伤口愈合。在水凝胶中培养的 hASCs 表现出增强的旁分泌活性,同时肝细胞生长因子的分泌增加。在切除全层皮肤伤口的糖尿病小鼠模型中,用 hASCs-水凝胶复合物治疗的小鼠表现出加速的伤口闭合和增加的新生血管形成。我们的研究结果表明,开发的 hDAM 水凝胶可以为 hASCs 提供有利的微环境,增强再生潜力以加速慢性伤口愈合。在水凝胶中培养的 hASCs 表现出增强的旁分泌活性,同时肝细胞生长因子的分泌增加。在切除全层皮肤伤口的糖尿病小鼠模型中,用 hASCs-水凝胶复合物治疗的小鼠表现出加速的伤口闭合和增加的新生血管形成。我们的研究结果表明,开发的 hDAM 水凝胶可以为 hASCs 提供有利的微环境,增强再生潜力以加速慢性伤口愈合。
更新日期:2020-11-30
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