Current work was designed to explore the effect of ZnO nanoparticles (ZnONP) biofabricated by using Trianthema portulacastrum (TP) leaves extract on mice brain hippocampus. ZnO nanoparticles of TP leaves (ZnOTP) were synthesized by co-precipitation method and further characterized by using various techniques such as UV-Vis spectrophotometer, Field Emission Scanning Electron Microscopy (FESEM), Fourier Transform Infrared (FTIR), and Energy Dispersive X-ray (EDX). ZnOTP were evaluated for in vitro antioxidant activity, in vivo behavior models (for assessment of cognitive ability), acetylcholinesterase (AChE) activity along with other neurotransmitters content determination, estimation of various oxidative stress parameters and analysis of zinc content in the brain as well as plasma. Histopathological evaluation of the brain hippocampus of each group was performed to corroborate the statistical results. Spherical ZnOTP of 10 to 20 nm size embedded with different phytoconstituents of TP was confirmed. Results of our study revealed a significant memory deficit in mice treated with ZnOTP. Neuronal degeneration was also observed via a significant increase in AChE activity and oxidative stress levels in the brain of mice administered with ZnOTP. Exposure of ZnOTP was also found responsible for modulation of neurotransmission in hippocampus area. Further, ZnOTP disturbed the zinc homeostasis in hippocampus via elevation of zinc content in brain as well as plasma. Histopathology of hippocampus supported the damaging impact of ZnOTP by an increase in vacuolated cytoplasm and focal gliosis in groups treated with ZnOTP. Results demonstrated the neurotoxic effect of ZnOTP on brain hippocampus via cognitive impairment by alteration of neurotransmitter level, zinc content and oxidative stress.

中文翻译：

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生物制造的氧化锌纳米颗粒通过调节小鼠的氧化应激和乙酰胆碱酯酶水平来损害认知功能

目前的工作旨在探索使用马齿苋 (TP) 叶提取物生物制造的 ZnO 纳米粒子 (ZnONP) 对小鼠大脑海马的影响。通过共沉淀法合成了 TP 叶片的 ZnO 纳米粒子 (ZnOTP)，并使用各种技术进一步表征，如紫外-可见分光光度计、场发射扫描电子显微镜 (FESEM)、傅里叶变换红外 (FTIR) 和能量色散 X-射线 (EDX)。对 ZnOTP 的体外抗氧化活性、体内行为模型（用于评估认知能力）、乙酰胆碱酯酶 (AChE) 活性以及其他神经递质含量的测定、各种氧化应激参数的估计和大脑中锌含量的分析进行了评估等离子体。对每组的大脑海马进行组织病理学评估以证实统计结果。确认了 10 至 20 nm 大小的球形 ZnOTP，其中嵌入了不同的 TP 植物成分。我们的研究结果揭示了用 ZnOTP 治疗的小鼠的显着记忆缺陷。通过施用 ZnOTP 的小鼠大脑中 AChE 活性和氧化应激水平的显着增加，还观察到神经元变性。还发现 ZnOTP 的暴露负责调节海马区的神经传递。此外，ZnOTP 通过提高大脑和血浆中的锌含量来扰乱海马中的锌稳态。海马的组织病理学通过在用 ZnOTP 治疗的组中空泡化的细胞质和局灶性神经胶质增生的增加来支持 ZnOTP 的破坏性影响。