Abstract Proteins derived from plant resources such as oilseed meals, canola and sunflower, are considered a viable alternative to animal proteins for food consumption. This work presents a rational methodology, using high throughput experimentation (HTE), for the separation of cruciferin and napin, the two major proteins of canola meal, by chromatography. Eight different mixed mode and ion exchange resins were evaluated at different conditions with the aim of capturing napin and identifying adsorption/desorption behavior, ease of desorption and selectivity. POROS 50 HS resulted as the most promising resin. The obtained equilibrium adsorption data for napin and cruciferin was used in a mechanistic chromatography model and compared with experimental results showing a very good agreement. The model was used to identify column operating parameters that lead to > yield and purity for both proteins. Subsequently a conceptual downstream processing was proposed.

中文翻译：

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用离子交换色谱法纯化油菜籽蛋白napin和cruciferin的高通量工艺开发

摘要 来自植物资源（如油籽粉、油菜籽和向日葵）的蛋白质被认为是用于食品消费的动物蛋白质的可行替代品。这项工作提出了一种合理的方法，使用高通量实验 (HTE)，通过色谱法分离十字花科素和油菜籽这两种菜籽粕的主要蛋白质。在不同条件下对八种不同的混合模式和离子交换树脂进行了评估，目的是捕获napin 并确定吸附/解吸行为、解吸的容易程度和选择性。POROS 50 HS 是最有前途的树脂。将获得的油菜籽和十字花科素的平衡吸附数据用于机械色谱模型，并与实验结果进行比较，显示出非常好的一致性。该模型用于确定导致两种蛋白质 > 产量和纯度的色谱柱操作参数。随后提出了概念性下游处理。