Multidrug resistant bacterial infections are difficult to treat and contribute to high morbidity and mortality. The phage vB PaeP-SaPL was isolated from a sewage drain (Lahore, Pakistan) against Pseudomonas aeruginosa PA-1 (NCBI Accession number MG763232). SaPL produced circular, transparent plaques, 4−5 mm in diameter and showed broad host range infecting 57 % of tested MDR P. aeruginosa clinical isolates (N = 38), while no infectivity was observed against any tested strains of other genera.

SaPL inhibited PA-1 growth until 24 h post infection at MOI of 1. The SaPL showed stability at varying temperature and pH, with optimum stability at pH 7 and 45 °C. The latent period of SaPL was 20 min with burst size of 155 virions. The genome of SaPL was double stranded DNA of 45,796 bps having 63 CDS (13 for known proteins and 50 for hypothetical proteins) with a GC content of 52 %. The termini analysis revealed that SaPL genome ends are redundant and permuted. The packaging strategy used by SaPL was a headful (pac) strategy like P1 phage. Survivability of PA-1 challenged mice, treated with SaPL (100 %) was statistically significant (P < 0.05) than in untreated challenged mice (0%). Based on its efficacy in reducing bacterial growth, selective infectivity against majority of P. aeruginosa strains and its ability to increase survivability in PA-1 challenged mice, SaPL is proposed to be a potential candidate for bacteriophage therapy against difficult to treat MDR P. aeruginosa infections.

耐多药细菌感染难以治疗并导致高发病率和死亡率。噬菌体 vB PaeP- SaPL从污水排放管（拉合尔，巴基斯坦）中分离出来，以对抗铜绿假单胞菌 PA-1（NCBI 登录号 MG763232）。SaPL产生圆形、透明的斑块，直径为 4-5 毫米，并显示出广泛的宿主范围，可感染 57% 的测试 MDR铜绿假单胞菌临床分离株（N = 38），而未观察到对其他属的任何测试菌株的感染性。

SaPL在 MOI 为 1 时抑制PA-1生长直到感染后 24 小时。SaPL在不同温度和 pH 值下表现出稳定性，在 pH 值 7 和 45°C 下具有最佳稳定性。潜伏期SAPL为20分钟为155个病毒粒子突发大小。SaPL的基因组是 45,796 bps 的双链 DNA，具有 63 个 CDS（已知蛋白质为 13 个，假设蛋白质为 50 个），GC 含量为 52%。末端分析显示SaPL基因组末端是冗余和排列的。SaPL使用的包装策略是像P1噬菌体一样的 headful ( pac ) 策略。PA-1 的生存能力用SaPL (100%) 处理的受攻击小鼠与未处理的受攻击小鼠 (0%) 相比具有统计学显着性 (P < 0.05)。基于其在减少细菌生长方面的功效、对大多数铜绿假单胞菌菌株的选择性感染性以及其在 PA-1 攻击小鼠中提高生存能力的能力，SaPL被提议作为噬菌体治疗难以治疗的 MDR铜绿假单胞菌的潜在候选物感染。