A novel portable and cost-effective multi-wavelength smartphone colorimetric reader (MW-SCR) was designed for the quantitation of liquid colorimetric assays. Six light-emitting diodes (LEDs) with different wavelengths were assembled in a roulette fashion to form the light detection source. An ambient light sensor of smartphone was applied as the detector. Using this MW-SCR, we detected six common serum analytes, including alkaline phosphatase(ALP), potassium(K⁺), chloride(Cl⁻), alanine aminotransaminase(ALT), glycated serum protein(GSP), and albumin, to evaluate the feasibility and reliability of the MW-SCR. Strong linear regression (R²>0.99) analysis with a wide linear range and good limits of detection were found for this device, detection limits as low as 0.37 U/L, 0.0083 mmol/L, 0.78 mmol/L, 4.49 KarU, 0.023 mmol/L and 2.23 g/L for ALP, K⁺,Cl⁻, ALT, GSP and albumin, respectively, were achieved. It also had high repeatability that ranged from 0.20 to 0.61 % relative standard deviations (RSD), and the precision ranged of MW-SCR were from 0.26 to 1.17 % RSDs. Results demonstrated that our device showed remarkable stability, accuracy, and repeatability, presenting excellent consistency with a professional microplate reader. It can be redesigned for multiple indications, such as clinical biochemical analyses, food safety testing, environmental monitoring and so on, because it is open source, user friendly and easy manufacturing.

一种新颖的便携式且具有成本效益的多波长智能手机比色读取器（MW-SCR）设计用于定量液体比色测定。以轮盘赌的方式组装了六个不同波长的发光二极管（LED）以形成光检测源。智能手机的环境光传感器被用作检测器。使用这种MW-SCR，我们检测到六种常见的血清的分析物，包括碱性磷酸酶（ALP），钾（K ⁺），氯（氯^- ），丙氨酸aminotransaminase（ALT），糖化血清蛋白（GSP），和白蛋白，以评价MW-SCR的可行性和可靠性。强线性回归（R ²> 0.99）分析，发现该设备具有宽线性范围和良好的检出限，检出限低至0.37 U / L，0.0083 mmol / L，0.78 mmol / L，4.49 KarU，0.023 mmol / L和2.23 g / L为ALP，K ⁺，氯^-分别获得了ALT，GSP和白蛋白。它还具有较高的重复性，相对标准偏差（RSD）为0.20至0.61％，MW-SCR的精密度范围为RSD的0.26至1.17％。结果表明，我们的设备显示出显着的稳定性，准确性和可重复性，与专业的酶标仪具有出色的一致性。由于它是开源的，用户友好的且易于制造的，因此可以针对多种适应症进行重新设计，例如临床生化分析，食品安全性测试，环境监测等。