Copper (Cu) is a trace mineral nutrient essential for plant growth; consequently, its uptake, trafficking, and redistribution are precisely controlled. Currently, little is known about the underlying regulatory mechanisms in rice. In this work, we identified OsHIPP24 as a Cu metallochaperone in rice. Heterologous expression of OsHIPP24 in wild-type yeast (Saccharomyces cerevisiae) strain BY4741 increased accumulation of Cu, as well as cadmium (Cd). OsHIPP24 was expressed constitutively in most tissues of rice, including root, stem, leaf, leaf sheath, internode, rachis, and spikelet, and particularly high in root stele along with the vascular bundle of the shoot. Overexpression or knockout of OsHIPP24 in rice led to stunted growth and reduced plant height, regardless of Cu levels in the growth medium. Taken together, these observations indicated that the physiological roles of OsHIPP24 involve the delivery of metal micronutrients via vascular tissues, and that OsHIPP24 plays a crucial role in the growth of rice.

铜是植物生长所必需的微量矿物质营养素。因此，对它的吸收，贩运和再分配进行了精确控制。目前，对稻米中潜在的调控机制知之甚少。在这项工作中，我们确定OsHIPP24为水稻中的Cu金属伴侣。的异源表达OsHIPP24在野生型酵母（酿酒酵母）菌株BY4741铜积累增加，以及镉（Cd）。OsHIPP24在水稻的大多数组织中组成性表达，包括根，茎，叶，叶鞘，节间，轴和小穗，特别是在根茎和脉管束中高表达。OsHIPP24的过表达或敲除不论生长培养基中的铜含量如何，水稻中的铜离子都会导致生长发育迟缓和株高降低。综上所述，这些观察结果表明，OsHIPP24的生理作用涉及通过血管组织传递金属微量营养素，并且OsHIPP24在水稻生长中起关键作用。