Papaver armeniacum hairy roots were induced by four Rhizobium rhizogenes strains on three explants (shoot, root, and hypocotyl). Also, the effects of two concentrations (100 and 200 μM) of methyl jasmonate (MJ) and salicylic acid (SA) were assessed on productions of papaverine, noscapine, thebaine, morphine, and codeine and expression of some related genes (TYDC, DBOX, BBE, SalAT, T6ODM, and COR) in P. armeniacum L. hairy root culture at 24 and 48 h after elicitation. R. rhizogenes strain C58C1 induced the highest hairy root rate on hypocotyl explant. Application of 100 μM MJ resulted in the highest contents of thebaine, codeine, and morphine by enhancing the expression of SalAT, COR, and T6ODM genes, respectively, while application of 100 μM SA resulted in the highest contents of papaverine and noscapine by upregulating DBOX and BBE genes, respectively. 100 μM MJ can be used as an effective elicitor in P. armeniacum hairy root culture to increase studied morphinan alkaloids. Also, SA can be suggested for enhancing papaverine and noscapine contents in P. armeniacum hairy root culture. It may be due to that there is a SA- and MJ-signaling crosstalk, which results in reciprocal antagonism between SA and MJ signaling pathways. The effects of MJ and SA elicitors on benzylisoquinoline alkaloids (BIAs) production were level-dependent.

罂粟亚美尼亚毛状根由三种外植体（芽，根和下胚轴）上的四个发根根霉菌株诱导。此外，评估了茉莉酸甲酯（MJ）和水杨酸（SA）两种浓度（100和200μM）对罂粟碱，芥子碱，蒂巴因，吗啡和可待因的产生以及某些相关基因（TYDC，DBOX）表达的影响，BBE，色拉，T6ODM，和COR）在杏黄兜在24 L.毛状根培养和诱导后48小时。根瘤菌C58C1菌株在下胚轴外植体上诱导了最高的毛根发生率。100μMMJ分别通过增强SalAT，COR和T6ODM基因的表达而导致蒂巴因，可待因和吗啡的含量最高，而100μMSA则通过上调DBOX导致罂粟碱和Noscapine的含量最高。和BBE基因。100μMMJ可用作亚美尼亚假单胞菌毛状根培养物中的有效引发剂，以增加已研究的吗啡生物碱。此外，可以建议使用SA来提高亚美尼亚假单胞菌中的罂粟碱和Noscapine含量。毛根培养。可能是由于存在SA和MJ信号的串扰，导致SA和MJ信号通路之间的相互拮抗。MJ和SA激发子对苄基异喹啉生物碱（BIAs）产生的影响是水平依赖性的。