Rhinopoma microphyllum is one of the species of bats that lives in arid and semi-arid areas of Iran. The initial suggestion of the presence of two subspecies R. m. microphyllum and R. m. harrisoni based on their morphological characteristics has been questioned on the basis of small differences between the populations. Later, other researchers assigned Iranian populations of this species to one or two subspecies based on their morphological and molecular characteristics. The present study provides a phylogeographical analysis of this species using 687 bp of the mitochondrial cytochrome b in 81 bats in Iran, Jordan, Levant and Ethiopia. Based on mtDNA sequences, we found a low degree of genetic diversity in the Iranian populations of R. microphyllum (π = 0.0025), which shows a close relationship between the haplotypes. The analysis of genetic distance (0.15-1.93%), phylogenetic trees, and statistical parsimony network showed that all Iranian samples were grouped in the same clade, while Levant, Jordan and Ethiopian samples belonged to a different clade. Molecular dating suggested the Iranian R. microphyllum lineage split from the R. microphyllum of the Levant and Jordan clade during the Pliocene 3.18 (2.11-4.32 Ma). Taking these results into consideration, we can conclude that all Iranian specimens belong to the same subspecies as R. m. harrisoni since molecular results indicate that Iranian samples are differ from Levant subspecies (R. m. microphyllum).

中文翻译：

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在伊朗的大鼠尾蝙蝠小叶犀牛（Chiroptera：Rhinopomatidae）中缺乏线粒体细胞色素 b 基因的种内变异

Rhinopoma microphyllum 是生活在伊朗干旱和半干旱地区的蝙蝠种类之一。存在两个亚种的初步建议 R. m. microphyllum 和 R. m. 由于种群之间的微小差异，harrisoni 因其形态特征而受到质疑。后来，其他研究人员根据形态和分子特征将这一物种的伊朗种群分配到一两个亚种。本研究使用伊朗、约旦、黎凡特和埃塞俄比亚的 81 只蝙蝠中 687 bp 的线粒体细胞色素 b 对该物种进行了系统地理学分析。基于 mtDNA 序列，我们发现伊朗小叶 R. (π = 0.0025) 种群的遗传多样性程度较低，这表明单倍型之间存在密切关系。对遗传距离（0.15-1.93%）、系统发育树和统计简约网络的分析表明，所有伊朗样本都属于同一进化枝，而黎凡特、约旦和埃塞俄比亚样本属于不同的进化枝。分子年代测定表明，在上新世 3.18 (2.11-4.32 Ma) 期间，伊朗的小叶小叶谱系从黎凡特和约旦进化枝的小叶小叶中分离出来。考虑到这些结果，我们可以得出结论，所有伊朗标本都与 R. m. 属于同一亚种。harrisoni，因为分子结果表明伊朗样本与黎凡特亚种（R. m. microphyllum）不同。分子年代测定表明，在上新世 3.18 (2.11-4.32 Ma) 期间，伊朗的小叶小叶谱系从黎凡特和约旦进化枝的小叶小叶中分离出来。考虑到这些结果，我们可以得出结论，所有伊朗标本都与 R. m. 属于同一亚种。harrisoni，因为分子结果表明伊朗样本与黎凡特亚种（R. m. microphyllum）不同。分子年代测定表明，在上新世 3.18 (2.11-4.32 Ma) 期间，伊朗的小叶小叶谱系从黎凡特和约旦进化枝的小叶小叶中分离出来。考虑到这些结果，我们可以得出结论，所有伊朗标本都与 R. m. 属于同一亚种。harrisoni，因为分子结果表明伊朗样本与黎凡特亚种（R. m. microphyllum）不同。