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Changes in cell wall structure and protein set in Candida maltosa grown on hexadecane
Folia Microbiologica ( IF 2.4 ) Pub Date : 2020-11-27 , DOI: 10.1007/s12223-020-00840-2
Anton Zvonarev 1 , Vasilina Farofonova 1 , Ekaterina Kulakovskaya 1 , Tatiana Kulakovskaya 1 , Andrey Machulin 1 , Svyatoslav Sokolov 2 , Vladimir Dmitriev 1
Affiliation  

The yeast Candida maltosa is a model organism for studying adaptive changes in the structure and function of the cell wall when consuming water-insoluble nutrient sources. The cells of C. maltosa that utilize hydrocarbons contain supramolecular structures, so-called “canals” in the cell wall. Differences in protein profiles of culture liquids and cell wall extracts of C. maltosa grown on glucose and hexadecane were analyzed. Three proteins specific of cells grown on hexadecane were revealed using mass spectrometry: glycosyl hydrolase EPD2 in the culture liquid; a protein belonging to the cytochrome C family in the 0.5 mol/L NaCl extract; and PPIA_CANAL protein known as chaperone, in the 0.1% SDS extract. The possible role of these proteins in cell wall structures responsible for adaptation to hexadecane utilization is discussed.



中文翻译:

在十六烷上生长的麦芽糖假丝酵母细胞壁结构和蛋白质组的变化

酵母假丝酵母麦芽糖是用于消耗的水不溶性营养源时在细胞壁的结构和功能的研究适应性变化的模型生物。利用碳氢化合物的C. maltosa细胞包含超分子结构,即细胞壁中所谓的“运河”。麦芽糖酵母培养液和细胞壁提取物蛋白质谱的差异分析了生长在葡萄糖和十六烷上的 。使用质谱法揭示了在十六烷上生长的细胞的三种特异性蛋白质:培养液中的糖基水解酶EPD2;0.5 mol/L NaCl 提取物中属于细胞色素 C 家族的蛋白质;和 PPIA_CANAL 蛋白称为分子伴侣,在 0.1% SDS 提取物中。讨论了这些蛋白质在负责适应十六烷利用的细胞壁结构中的可能作用。

更新日期:2020-12-01
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