Collagen has been widely shown to promote osteogenesis of bone marrow mesenchymal stromal cells (BM-MSCs). Due to the invasive procedure of obtaining BM-MSCs, MSCs from other tissues have emerged as a promising alternative for regenerative therapy. MSCs originated from different sources, exhibiting different differentiation potentials. Therefore, the applicability of collagen type I, combining with amniotic membrane (AM)-MSCs was examined through proliferation and differentiation assays together with the expression of surface markers and genes associated with stemness and differentiation under basal or induction conditions. No increase in cell growth was observed because AM-MSCs might be directed towards spontaneous osteogenesis. This was evidenced by the calcium deposition and elevated expression of osteogenic genes when AM-MSCs were cultured in collagen plate with basal media. Under the osteogenic condition, reciprocal expression of OCN and CEBPA suggested a shift towards adipogenesis. Surprisingly, adipogenic genes were not elevated upon adipogenic induction, although oil droplets deposition was observed. In conclusion, our findings demonstrated that collagen causes spontaneous osteogenesis in AM-MSCs. However, the presence of exogenous inductors could shift the direction of adipo-osteogenic gene regulatory network modulated by collagen.

中文翻译：

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I型胶原蛋白促进基础和诱导培养基中羊膜来源的间质基质细胞的成骨分化。

胶原蛋白已被广泛证明可促进骨髓间质基质细胞（BM-MSC）的成骨。由于获得BM-MSC的侵入性过程，来自其他组织的MSC已经成为再生疗法的有希望的替代物。MSC来自不同来源，表现出不同的分化潜力。因此，在基础或诱导条件下，通过增殖和分化测定以及与干和分化相关的表面标志物和基因的表达，研究了I型胶原与羊膜（AM）-MSC结合的适用性。没有观察到细胞生长的增加，因为AM-MSC可能是针对自发成骨的。当在基础培养基的胶原板中培养AM-MSC时，钙沉积和成骨基因的表达升高证明了这一点。在成骨条件下，OCN和CEBPA的相互表达提示向脂肪形成的转变。出人意料的是，尽管观察到油滴沉积，但在成脂诱导后成脂基因并未升高。总之，我们的发现表明胶原蛋白可引起AM-MSCs自发成骨。然而，外源性诱导剂的存在可能会改变由胶原蛋白调节的脂肪成骨基因调控网络的方向。尽管观察到油滴沉积。总之，我们的发现表明胶原蛋白可引起AM-MSCs自发成骨。然而，外源性诱导剂的存在可能会改变由胶原蛋白调节的脂肪成骨基因调控网络的方向。尽管观察到油滴沉积。总之，我们的发现表明胶原蛋白可引起AM-MSCs自发成骨。然而，外源性诱导剂的存在可能会改变由胶原蛋白调节的脂肪成骨基因调控网络的方向。