Ovarian cancer (OC) is a commonly diagnosed female cancer. Ligustrazine (LSZ), a natural compound, has been reported to exert anti-cancer activity, although the mechanisms underlying the anticancer effects are not clear. This study investigated the impact of LSZ on cell proliferation and migration by regulating mircorna-211(miR-211) expression using the human ovarian cancer SK-OV-3 and OVCAR-3 cell lines. OC cells were treated with 0, 0.5, 1, and 2 mM LSZ, and quantitative real-time PCR was utilized to measure miR-211 levels in SK-OV-3 and OVCAR-3 cells with different treatment. Moreover, to further confirm the roles of miR-211 in LSZ induced anti-tumor effects, miR-211 expression was inhibited by transfection of miR-211 inhibitors in SK-OV-3 cells. Cell proliferation of transfected cells was evaluated using the CCK-8 and colony formation assay. The scratch assay was employed to assess cell migration and transwell assay was performed for evaluating the cell invasion. Protein levels of epithelial-mesenchymal transition (EMT) markers were determined by western blotting. We found that LSZ inhibited the viability, proliferation, migration and invasion ability of SK-OV-3 and OVCAR-3 cells in a dose-dependent manner; moreover, LSZ could significantly increase the expression of miR-211 in both SK-OV-3 and OVCAR-3, and knockdown of miR-211 in SK-OV-3 cells partially abrogated the anti-tumor behavior of LSZ by promoting the viability, proliferation, migration, invasion and EMT of SK-OV-3 cells. Thus, we found that Ligustrazine can inhibit the proliferation and migration of ovarian cancer cells via regulating miR-211. Our study suggests that LSZ might be a potential and effective treatment for OC.

中文翻译：

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川芎嗪通过调节 miR-211 抑制卵巢癌细胞的增殖和迁移。

卵巢癌 (OC) 是一种常见的女性癌症。据报道，川芎嗪 (LSZ) 是一种天然化合物，具有抗癌活性，但其抗癌作用的机制尚不清楚。本研究通过使用人卵巢癌 SK-OV-3 和 OVCAR-3 细胞系调节 mircorna-211(miR-211) 表达来研究 LSZ 对细胞增殖和迁移的影响。OC 细胞用 0、0.5、1 和 2 mM LSZ 处理，并利用实时定量 PCR 来测量不同处理的 SK-OV-3 和 OVCAR-3 细胞中的 miR-211 水平。此外，为了进一步证实 miR-211 在 LSZ 诱导的抗肿瘤作用中的作用，通过在 SK-OV-3 细胞中转染 miR-211 抑制剂来抑制 miR-211 的表达。使用 CCK-8 和集落形成试验评估转染细胞的细胞增殖。划痕试验用于评估细胞迁移，transwell 试验用于评估细胞侵袭。通过蛋白质印迹确定上皮间质转化 (EMT) 标记的蛋白质水平。我们发现LSZ以剂量依赖性方式抑制SK-OV-3和OVCAR-3细胞的活力、增殖、迁移和侵袭能力；此外，LSZ 可显着增加 SK-OV-3 和 OVCAR-3 中 miR-211 的表达，并且敲低 SK-OV-3 细胞中的 miR-211 通过提高细胞活力部分消除了 LSZ 的抗肿瘤行为SK-OV-3 细胞的增殖、迁移、侵袭和 EMT。因此，我们发现川芎嗪可以通过调节 miR-211 来抑制卵巢癌细胞的增殖和迁移。我们的研究表明，LSZ 可能是治疗 OC 的一种潜在且有效的方法。