The diamondback moth (Plutella xylostella L.) (Lepidoptera: Plutellidae) is one of the major pests in cabbage which causes severe loss to the cruciferous crops. Entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae have been established as endophytes in cabbage plants by seed treatment/root inoculation/foliar application methods in glasshouse conditions. A glasshouse experiment was conducted to study the effect of endophytic isolates of B. bassiana (NBAIR Bb-5a and NBAIR Bb-45) and M. anisopliae (NBAIR Ma-4 and NBAIR Ma-35) on P. xylostella in cabbage using detached leaf bioassay method. The isolates were applied through seed treatment/root inoculation/foliar application at the concentration of 1 × 108 spores/ml and evaluated at 15, 30, 45, and 60 days after treatment (DAT) in 2017 and 2018. These isolates were colonized in cabbage leaf tissues from 15 to 60 DAT. All 4 isolates showed different mortality percentages during 15–30 DAT, and no mortality was observed after 45 and 60 DAT in different inoculation methods during both years. Among the application methods tested, foliar application method gave the highest mortality of 70–80% at 15 DAT and 12–58% at 30 DAT mortality of 2nd instar larvae of P. xylostella in cabbage. Among the isolates tested, NBAIR Ma-35 showed the highest mortality (35–79%) in all the 3 inoculation methods tested. Endophytic isolates of B. bassiana and M. anisopliae suppressed the population of P. xylostella on cabbage leaves in three inoculation methods tested. Among all the methods tested, foliar application method showed highest mortality. These promising isolates have to be further tested under field conditions for management of P. xylostella in cabbage.

中文翻译：

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白僵菌（Balveria bassiana（Balsamo）Vuillemin和Metarhizium anisopliae（Metchnikoff）Sorokin ）的内生分离物对小白菜小菜蛾（鳞翅目：Plutellidae）的影响

小菜蛾（Plutella xylostella L.）（鳞翅目：Plutellidae）是卷心菜中的主要害虫之一，导致十字花科作物严重损失。通过在温室条件下的种子处理/根系接种/叶面施用方法，已将病原性真菌球孢白僵菌和无核白僵菌建立为甘蓝植物中的内生菌。进行了一个温室实验，研究了球芽孢杆菌（NBAIR Bb-5a和NBAIR Bb-45）和分枝分枝杆菌（NBAIR Ma-4和NBAIR Ma-35）的内生分离物对菜青菜中小菜蛾的影响。叶生物测定法。通过种子处理/根接种/叶面施用以1×108孢子/ ml的浓度应用分离株，并在2017年和2018年处理后（DAT）15、30、45和60天进行评估。这些分离物定植于15至60 DAT的甘蓝叶组织中。在15–30 DAT期间，所有4种分离株均显示出不同的死亡率百分比，并且在这两种年份中，在不同接种方法下分别进行45和60 DAT后均未观察到死亡率。在测试的施用方法中，叶面施用方法在白菜假单胞菌第二龄幼虫的15 DAT时死亡率最高，在30 DAT时为12–58％。在测试的分离株中，NBAIR Ma-35在所有3种接种方法中均显示出最高的死亡率（35–79％）。在三种测试接种方法中，球孢杆菌和拟南芥的内生分离株均能抑制白菜叶片上小菜蛾的种群。在所有测试方法中，叶面施用方法显示出最高的死亡率。