Bacterial cultures allow the identification of infectious disease pathogens. However, obtaining the results of conventional culture methods is time-consuming, taking at least two days. A more efficient alternative is the use of concentrated bacterial samples to accelerate culture growth. Our study focuses on the development of a high-yield sample concentrating technique. A total of 71 paired samples were obtained from patients on peritoneal dialysis (PD). The peritoneal dialysates were repeat-centrifuged and then washed with saline, namely the centrifuging and washing method (C&W method). The concentrated samples were Gram-stained and inoculated into culture plates. The equivalent unprocessed dialysates were cultured as the reference method. The times until culture results for the two methods were compared. The reference method yielded no positive Gram stain results, but the C&W method immediately gave positive Gram stain results for 28 samples (p < 0.001). The culture-negative rate was lower in the C&W method (5/71) than in the reference method (13/71) (p = 0.044). The average time for bacterial identification achieved with the C&W method (22.0 h) was shorter compared to using the reference method (72.5 h) (p < 0.001). The C&W method successfully concentrated bacterial samples and superseded blood culture bottles for developing adequate bacterial cultures. The C&W method may decrease the culture report time, thus improving the treatment of infectious diseases.

中文翻译：

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反复离心和洗涤可浓缩腹膜透析中的细菌样本以获得最佳培养：原创文章

细菌培养允许鉴定传染病病原体。然而，获得常规培养方法的结果非常耗时，至少需要两天时间。更有效的替代方法是使用浓缩的细菌样本来加速培养物的生长。我们的研究重点是开发高产样品浓缩技术。从腹膜透析 (PD) 患者中获得了总共 71 个配对样本。腹膜透析液重复离心后用生理盐水洗涤，即离心洗涤法（C&W法）。将浓缩样品进行革兰氏染色并接种到培养板中。培养等效的未处理透析液作为参考方法。比较两种方法培养结果的时间。参考方法没有产生阳性革兰氏染色结果，但 C&W 方法立即给出了 28 个样品的阳性革兰氏染色结果 (p < 0.001)。C&W 方法中的培养阴性率 (5/71) 低于参考方法 (13/71) (p = 0.044)。与使用参考方法 (72.5 小时) 相比，使用 C&W 方法 (22.0 小时) 实现的细菌识别平均时间更短 (p < 0.001)。C&W 方法成功地浓缩了细菌样本并取代了血培养瓶，以开发足够的细菌培养物。C&W方法可以减少培养报告时间，从而改善传染病的治疗。C&W 方法中的培养阴性率 (5/71) 低于参考方法 (13/71) (p = 0.044)。与使用参考方法 (72.5 小时) 相比，使用 C&W 方法 (22.0 小时) 实现的细菌识别平均时间更短 (p < 0.001)。C&W 方法成功地浓缩了细菌样本并取代了血培养瓶，以开发足够的细菌培养物。C&W方法可以减少培养报告时间，从而改善传染病的治疗。C&W 方法中的培养阴性率 (5/71) 低于参考方法 (13/71) (p = 0.044)。与使用参考方法 (72.5 小时) 相比，使用 C&W 方法 (22.0 小时) 实现的细菌识别平均时间更短 (p < 0.001)。C&W 方法成功地浓缩了细菌样本并取代了血培养瓶，以开发足够的细菌培养物。C&W方法可以减少培养报告时间，从而改善传染病的治疗。W 方法成功地浓缩了细菌样本并取代了血培养瓶，以开发足够的细菌培养物。C&W方法可以减少培养报告时间，从而改善传染病的治疗。W 方法成功地浓缩了细菌样本并取代了血培养瓶，以开发足够的细菌培养物。C&W方法可以减少培养报告时间，从而改善传染病的治疗。