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QTL Analysis and Fine Mapping of a Major QTL Conferring Kernel Size in Maize (Zea mays)
Frontiers in Genetics ( IF 2.8 ) Pub Date : 2020-11-05 , DOI: 10.3389/fgene.2020.603920
Guiying Wang 1 , Yanming Zhao 1, 2 , Wenbo Mao 1 , Xiaojie Ma 1 , Chengfu Su 1, 2
Affiliation  

Kernel size is an important agronomic trait for grain yield in maize. The purpose of this study is to map QTLs and predict candidate genes for kernel size in maize. A total of 199 F2 and its F2:3 lines from the cross between SG5/SG7 were developed. A composite interval mapping (CIM) method was used to detect QTLs in three environments of F2 and F2:3 populations. The result showed that a total of 10 QTLs for kernel size were detected, among which were five QTLs for kernel length (KL) and five QTLs for kernel width (KW). Two stable QTLs, qKW-1, and qKL-2, were mapped in all three environments. Three QTLs, qKL-1, qKW-1, and qKW-2, were overlapped with the QTLs identified from previous studies. In order to validate and fine map qKL-2, near-isogenic lines (NILs) were developed by continuous backcrossing between SG5 as the donor parent and SG7 as the recurrent parent. Marker-assisted selection was conducted from BC2F1 generation with molecular markers near qKL-2. A secondary linkage map with six markers around the qKL-2 region was developed and used for fine mapping of qKL-2. Finally, qKL-2 was confirmed in a 1.95 Mb physical interval with selected overlapping recombinant chromosomes on maize chromosome 9 by blasting with the Zea_Mays_B73 v4 genome. Transcriptome analysis showed that a total of 11 out of 40 protein-coding genes differently expressed between the two parents were detected in the identified qKL-2 interval. GRMZM2G006080 encoding a receptor-like protein kinase FERONIA, was predicted as a candidate gene to control kernel size. The work will not only help to understand the genetic mechanisms of kernel size of maize but also lay a foundation for further fine mapping and even cloning of the promising loci.



中文翻译:

玉米 (Zea mays) 赋予籽粒大小的主要 QTL 的 QTL 分析和精细定位

籽粒大小是玉米籽粒产量的重要农艺性状。本研究的目的是绘制 QTL 图谱并预测玉米籽粒大小的候选基因。来自SG5/SG7之间杂交的总共199个F 2及其F 2 : 3品系被开发出来。采用复合区间作图(CIM)方法检测F 2和F 2 : 3群体三种环境中的QTL。结果表明,共检测到10个与籽粒大小相关的QTL,其中5个与籽粒长度(KL)相关的QTL,5个与籽粒宽度(KW)相关的QTL。两个稳定的QTL,qKW-1, 和qKL-2,在所有三个环境中进行了映射。三个QTL,qKL-1, qKW-1, 和qKW-2,与之前研究中确定的 QTL 重叠。为了验证和精细地图qKL-2,近等基因系(NIL)是通过 SG5 作为供体亲本和 SG7 作为轮回亲本之间的连续回交而开发的。从 BC 2 F 1代开始进行标记辅助选择,分子标记接近qKL-2。二级连锁图谱周围有六个标记qKL-2区域已开发并用于精细测绘qKL-2。最后,qKL-2通过用 Zea_Mays_B73 v4 基因组进行爆破,在玉米 9 号染色体上选择重叠重组染色体,在 1.95 Mb 物理间隔中证实了这一点。转录组分析显示,在鉴定的 40 个蛋白质编码基因中,在两个亲本之间总共检测到 11 个差异表达的基因。qKL-2间隔。GRMZM2G006080 编码受体样蛋白激酶 FERONIA,被预测为控制籽粒大小的候选基因。这项工作不仅有助于了解玉米籽粒大小的遗传机制,而且为进一步精细定位甚至克隆有希望的位点奠定基础。

更新日期:2020-11-27
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