Abstract

Aim: This paper aims to study in-vitro and in-vivo evaluation of chitosan (CHI) biocomposite of gentamicin nanoparticles (GNPs) for wound healing.

Methods: In this study, CHI nanoparticles (NPs) were prepared using the ionic gelation technique. GNP biocomposites were examined on the excision wound model in Wistar rats to determine the in-vivo efficiency.

Results: The diameter and zeta potential of NPs were between 151–212.9 nm and 37.2 − 51.1 mV, respectively. The entrapment efficiency was in an acceptable range of 36.6–42.7% w/w. The release test information was fitted to mathematical models (Zero, First order, Higuchi, and Korsmeyer–Peppas), and according to calculations, the kinetics of drug release followed the Korsmeyer–Peppas model. A comparison of thermograms revealed that the drug was present in the formulation in a non-crystalline form.

Conclusion: Histological studies of the wound showed that the rate of skin tissue repair was higher in the GNP biocomposite treatment group than in the others.

 抽象的

目的：本文旨在研究庆大霉素纳米颗粒（GNP）的壳聚糖（CHI）生物复合材料用于伤口愈合的体外和体内评价。

方法：在本研究中，使用离子凝胶技术制备了 CHI 纳米颗粒（NP）。在 Wistar 大鼠的切除伤口模型上检查 GNP 生物复合材料以确定体内效率。

结果：纳米粒子的直径和zeta电位分别在151-212.9 nm和37.2-51.1 mV之间。包封率在 36.6-42.7% w/w 的可接受范围内。将释放测试信息拟合到数学模型（零、一阶、Higuchi 和 Korsmeyer-Peppas），根据计算，药物释放动力学遵循 Korsmeyer-Peppas 模型。热分析图的比较表明药物以非晶体形式存在于制剂中。

结论：伤口的组织学研究表明，GNP 生物复合材料治疗组的皮肤组织修复率高于其他组。