Metabolomics was used to study the influences of smoke-water (SW) and karrikinolide (KAR₁) on the biosynthesis of flavonoids and terpenoids in Salvia miltiorrhiza Bunge. The results showed that a total of 178 and 199 differential metabolites were obtained in SW and KAR_1, respectively, compared to the control. The differential metabolites were assigned to the corresponding metabolic pathways. The results indicated that some metabolic pathways in treatments of SW and KAR₁ overlapped, suggesting that treatments of SW and KAR₁ showed similar effects on the metabolic mechanism of S. miltiorrhiza. To obtain a clear overview of changes in metabolic regulation, TCA cycle, glycolytic pathway, biosynthesis of flavonoids and terpenoids and amino acids metabolism pathway were mapped into a network. We found that treatments with SW and KAR₁ could significantly promote the biosynthesis of flavonoids and terpenoids in S. miltiorrhiza. This study could help us better understand the influences of SW and KAR₁ on secondary metabolites and their underlying mechanism.

代谢组学被用于研究烟水（SW）和karrikinolide（KAR ₁）对丹参中黄酮类和萜类化合物生物合成的影响。结果显示，与对照组相比，SW和KAR ₁分别获得了178和199种差异代谢产物。将差异代谢物分配给相应的代谢途径。结果表明，SW和KAR _1的一些代谢途径重叠，这表明SW和KAR _1的治疗对S. miltiorrhiza的代谢机制具有相似的作用。。为了清楚地了解代谢调节的变化，将TCA循环，糖酵解途径，类黄酮和萜类化合物的生物合成以及氨基酸代谢途径映射到网络中。我们发现有SW和KAR是治疗₁可显著促进类黄酮和萜类化合物的生物合成丹参。这项研究可以帮助我们更好地了解SW和KAR ₁对次级代谢产物的影响及其潜在机制。