Clarifying the bacterial community composition and function in alkali soils is pivotal to the soil capability of sustaining venerable ecosystems. Using MiSeq sequencing, PICRUSt, and Biolog (https://www.biolog.com), we investigated the bacterial community composition and metabolic function of microbes in three soils differing in the severity of alkalinity. A high level of alkalinity deceased the diversity of microbial community and altered microbial community composition with the increase in relative abundances of 21 genera. The linear discriminate analysis and effect size analysis found most of 54 biomarkers belonged to Actinobacteria and Proteobacteria. Redundancy analysis indicated that the succession of bacterial community and metabolic characteristics of microbes were highly affected by the nutrient availability and soil moisture. The utilization rates of carbohydrates, amino acids, carboxylic acids, polymers, phenolic compound, and aminesides by microbes were positively correlated with soil nutrient concentrations but negatively correlated with the exchangeable sodium percentage and pH. Severe alkalinity inhibited the microbial community diversity and function on carbohydrate metabolisms.

中文翻译：

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草原碱度梯度改变土壤细菌群落组成和功能

澄清碱性土壤中的细菌群落组成和功能对于土壤维持古老生态系统的能力至关重要。使用MiSeq测序，PICRUSt和Biolog（https://www.biolog.com），我们研究了三种碱度不同的土壤的细菌群落组成和微生物的代谢功能。较高的碱度会降低微生物群落的多样性，并随着21属的相对丰度的增加而改变微生物群落的组成。线性判别分析和效应大小分析发现，54种生物标志物中的大多数属于放线菌和变形杆菌。冗余度分析表明，养分有效性和土壤水分对细菌群落的演替和微生物的代谢特性影响很大。微生物对碳水化合物，氨基酸，羧酸，聚合物，酚类化合物和胺类的利用率与土壤养分浓度呈正相关，而与可交换钠含量和pH呈负相关。严重的碱度抑制了微生物群落的多样性，并抑制了碳水化合物的代谢。