Experimental Findings about the Fluorescence Emission of Generation 4.0 and 4.5 Polyamidoamine Dendrimers,ChemistrySelect

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Experimental Findings about the Fluorescence Emission of Generation 4.0 and 4.5 Polyamidoamine Dendrimers
ChemistrySelect ( IF 1.9 ) Pub Date : 2020-11-27 , DOI: 10.1002/slct.202001474
David E. Ybarra _{1,

2} , Daniela E. Igartúa _{1,

2} , Silvia V. Alonso _{1,

2} , Fernando C. Alvira _{1,

2}

Affiliation

Physicochemical characterization of polyamidoamine (PAMAM) dendrimers of generation 4.0 amine‐terminated (DG4.0) and 4.5 carboxy‐ended (DG4.5) was done. We have measured the pKa of the inner tertiary amine, the surface primary‐amine, and carboxyl‐terminal groups. We have conducted UV‐Vis absorption and fluorescence emission experiments as a function of pH. We have made a 4th derivative analysis of the UV‐Vis absorption experiments and compare the results with classical amide such as dimethylformamide. Our results have permitted us to calculate the pKa of the groups sensible to the pH in both dendrimers and compare the results with theoretical studies. On the one hand, the pKa values found for DG4.0 were 10.0±0.5 and 7.1±0.2, for primary and tertiary amines groups, respectively. On the other hand, the pKa values found for the DG4.5 were 3.5±0.7 and 6.8±0.4, for the carboxylic and tertiary amine groups. Our experimental results agree with theoretical ones. We offer probes that the dendrimers have two non‐traditional fluorophores, where one of them is the inner amide bond. The dendrimers show fluorescence emission in all the tested pH.

中文翻译：

关于4.0和4.5代聚酰胺酰胺树状大分子荧光发射的实验结果

进行了4.0胺端（DG4.0）和4.5羧基端（DG4.5）的聚酰胺型（PAMAM）树状聚合物的理化表征。我们测量了内部叔胺，表面伯胺和羧基末端基团的pKa。我们进行了UV-Vis吸收和荧光发射实验，作为pH的函数。我们对UV-Vis吸收实验进行了四阶导数分析，并将结果与经典酰胺（例如二甲基甲酰胺）进行了比较。我们的结果使我们能够计算出对两种树枝状聚合物的pH敏感的基团的pKa，并将结果与理论研究进行比较。一方面，对于伯胺和叔胺基团，DG4.0的pKa值分别为10.0±0.5和7.1±0.2。另一方面，对于DG4.5发现的pKa值为3.5±0。对于羧基和叔胺基团为7和6.8±0.4。我们的实验结果与理论一致。我们提供的树枝状聚合物具有两个非传统荧光团的探针，其中一个是内部酰胺键。树枝状聚合物在所有测试的pH下均显示荧光发射。

更新日期：2020-11-27

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