The redox fluctuations regulated by endogenous reactive oxygen species and reductive sulphur species play vital roles in many biological functions. Simultaneously, it has been discovered that HOCl and H₂S are able to interfere with calcium homeostasis via the regulation of calcium channels in neurons. Therefore, a novel fluorescent probe PzSe containing a buit-in selenium atom as reversible responding site has been designed and synthesized. The selenium atom in PzSe can be oxidized to the form of selenoxide by HOCl, and the oxidized form of selenoxide can be reduced rapidly back to its original form of selenide by H₂S. The conversion of the probe between its selenoxide and selenide forms lead the fluophore to present electron-withdrawing and electron-rich effects, respectively, which lead to the probe present different optical properties at the oxidized and reduced states. Based on the rapid valence-guided reversible molecular events, PzSe could sensitively and selectively detect the HOCl/H₂S redox cycle in the solution and in living cells. Meanwhile, via the reversible three-colour fluorescence changes from the PC-12 cells being co-stained with the probe PzSe and the Ca²⁺-selective Fluo-4 AM, it can be used for trapping the concentration-dependent dynamic correlations among HOCl, H₂S, and Ca²⁺ in neurons.

内源性活性氧和还原性硫调节的氧化还原起伏在许多生物学功能中起着至关重要的作用。同时，已经发现HOCl和H ₂ S能够通过调节神经元中的钙通道来干扰钙稳态。因此，已经设计并合成了一种新的荧光探针PzSe，它含有一个硒原子作为可逆响应位点。PzSe中的硒原子可以被HOCl氧化成硒化形式，硒化氢的氧化形式可以被H ₂迅速还原成硒化物的原始形式S.探针在其硒氧化物和硒化物形式之间的转化导致发荧光团分别呈现吸电子和富电子效应，这导致探针在氧化态和还原态呈现不同的光学性质。基于快速价导的可逆分子事件，PzSe可以灵敏和选择性地检测溶液和活细胞中的HOCl / H ₂ S氧化还原循环。同时，通过与探针PzSe和Ca ²⁺选择性Fluo-4 AM共染色的PC-12细胞可逆的三色荧光变化，可用于捕获HOCl之间的浓度依赖性动态相关性，H ₂ S和Ca ²⁺ 在神经元中。