Abstract Temperature is an essential parameter in various fields, including chemistry, biology, environmentology, industry, and medical sciences. Currently, temperature detection using practical colorimetric methods has not been sufficiently explored. In this study, we established a polyethylene glycol-functionalized graphene oxide (PEG-GO)-based colorimetric thermosensor using guanine (G)-rich DNAzyme (Dz) and peptide nucleic acid (PNA). Dz served as the template DNA for thermosensitive nanostructures and as catalytic DNA for colorimetric assays. The peroxidase-like activity of Dz was completely inhibited after hybridizing with PNA, which could be recovered in the presence of PEG-GO in a thermosensitive manner, resulting in colorimetric temperature visualization. The temperature-sensing range of this system could be simply tuned by designing a PNA strand based on the melting temperature of the Dz/PNA duplex of interest. Smartphone assistance in this colorimetric platform enabled more precise, sensitive, and practical recognition of fine temperature changes. Moreover, this design permitted recall of the target temperature, enabling the visualization of the temperature at a later point. This robust system has the potential to be a tool for rapid temperature-sensing applications, such as in health diagnostics and food safety, and it could become a valuable resource for basic and applied nanobiotechnology research.

中文翻译：

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用于记录温度的基于聚乙二醇化氧化石墨烯的比色传感器

摘要 温度是化学、生物学、环境学、工业和医学等各个领域的重要参数。目前，尚未充分探索使用实际比色法进行温度检测。在这项研究中，我们使用富含鸟嘌呤 (G) 的 DNA 酶 (Dz) 和肽核酸 (PNA) 建立了一种基于聚乙二醇功能化氧化石墨烯 (PEG-GO) 的比色热传感器。Dz 作为热敏纳米结构的模板 DNA 和比色分析的催化 DNA。Dz 的过氧化物酶样活性在与 PNA 杂交后被完全抑制，可以在 PEG-GO 存在下以热敏方式恢复，导致比色温度可视化。通过根据感兴趣的 Dz/PNA 双链体的熔化温度设计 PNA 链，可以简单地调整该系统的温度感应范围。这个比色平台中的智能手机辅助能够更精确、灵敏和实用地识别细微的温度变化。此外，这种设计允许调用目标温度，从而能够在稍后的时间点可视化温度。这种强大的系统有可能成为快速温度传感应用的工具，例如健康诊断和食品安全，它可能成为基础和应用纳米生物技术研究的宝贵资源。并实际识别细微的温度变化。此外，这种设计允许调用目标温度，从而能够在稍后的时间点可视化温度。这种强大的系统有可能成为快速温度传感应用的工具，例如健康诊断和食品安全，它可能成为基础和应用纳米生物技术研究的宝贵资源。并实际识别细微的温度变化。此外，这种设计允许调用目标温度，从而能够在稍后的时间点可视化温度。这种强大的系统有可能成为快速温度传感应用的工具，例如健康诊断和食品安全，它可能成为基础和应用纳米生物技术研究的宝贵资源。