Reducing the Campylobacter load on poultry carcasses represents a major tasks for the industry as its ability to reduce their presence is of major interest aiming to increase consumer safety. This study investigated the ability of a mixture of natural antimicrobials (A3001) to reduce the adherence of the T6SS^+/− C. coli isolates (NC1^hcp-, NC2 ^hcp- and NC3 ^hcp+) to chicken neck skin and whole carcasses. Overall, the antimicrobial mixture induced a significant reduction in the capability of our C. coli isolates to colonise the chicken skin (p < 0.05) and carcasses (p < 0.0001) but with a greater effect (≈3 log reduction) on the NC3 isolate. Using the HCT-8 in vitro infection model we also show that at a concentration of 0.5% A3001, the impact on the NC3 isolate is accompanied by the downregulation of the hcp gene (p = 0.0001), and indicator of the T6SS presence. The results described herein also indicated that these isolates are highly resistant to H₂O₂, up to 20 mM, suggesting a high resilience to environmental stresses. In summary our study shows that natural antimicrobials can reduce the ability of T6SS positive chicken C. coli isolates to adhere to chicken skin or to the whole carcass and to infect epithelial cells in vitro and could be considered a potential intervention at processor level.

减少家禽屠体的弯曲杆菌负荷是该行业的一项主要任务，因为减少其存在的能力是旨在提高消费者安全性的主要兴趣。本实验研究天然抗微生物剂（A300 1）的混合物中，以降低T6SS的粘附的能力^+/- C.大肠杆菌株（NC1 ^{HCP -}，NC2 ^{HCP -}和NC3 ^{HCP +}），以鸡颈部皮肤和整个屠体。总体而言，抗菌混合物导致我们大肠杆菌的能力大大降低分离株在鸡皮（p <0.05）和屠体（p <0.0001）上定殖，但对NC3分离株的影响更大（减少约3 log）。使用HCT-8体外感染模型，我们还显示浓度为0.5％的A3001，对NC3分离株的影响伴随hcp基因的下调（p = 0.0001）和T6SS存在的指示。本文所述的结果还表明，这些分离物对H ₂ O ₂具有高抗性，最高达20mM，表明对环境胁迫具有高的复原力。总而言之，我们的研究表明，天然抗菌剂可以降低T6SS阳性鸡大肠杆菌的能力。分离物粘附在鸡皮肤或整个the体上，并在体外感染上皮细胞，在加工者水平上可以认为是潜在的干预措施。