Aspergillus niger reproduces asexually by forming conidia. Here, the minimal nutrient requirements were studied that activate germination of A. niger conidia. To this end, germination was monitored in time using an oCelloScope imager. Data was used as input in an asymmetric model to describe the process of swelling and germ tube formation. The maximum number of spores (P_max) that were activated to swell and to form germ tubes was 32.54% and 20.51%, respectively, in minimal medium with 50 mM glucose. In contrast, P_max of swelling and germ tube formation was <1% in water or 50 mM glucose. Combining 50 mM glucose with either NaNO₃, KH₂PO4, or MgSO4 increased P_max of swelling and germination up to 15.25% and 5.4%, respectively, while combining glucose with two of these inorganic components further increased these P_max values up to 25.85% and 10.99%. Next, 10 mM amino acid was combined with a phosphate buffer and MgSO₄. High (e.g. proline), intermediate and low (e.g. cysteine) inducing amino acids were distinguished. Together, a combination of an inducing carbon source with either inorganic phosphate, inorganic nitrogen or magnesium sulphate is the minimum requirement for A. niger conidia to germinate.

黑曲霉通过形成分生孢子进行无性繁殖。在这里，研究了激活黑曲霉分生孢子萌发的最低营养需求。为此，使用 oCelloScope 成像仪及时监测发芽情况。数据被用作非对称模型的输入来描述膨胀和胚芽管形成的过程。在含有 50 mM 葡萄糖的基本培养基中，被激活膨胀并形成胚管的最大孢子数 (P _max ) 分别为 32.54% 和 20.51%。相比之下，在水或 50 mM 葡萄糖中，肿胀和胚管形成的P _max小于 1%。将 50 mM 葡萄糖与 NaNO ₃、KH ₂ PO4 或 MgSO4 结合可提高 P _max膨胀和发芽率分别高达 15.25% 和 5.4%，同时将葡萄糖与这些无机成分中的两种组合进一步将这些 P_最大值提高到 25.85% 和 10.99%。接下来，将 10 mM 氨基酸与磷酸盐缓冲液和 MgSO _{4 混合}。区分高（例如脯氨酸）、中等和低（例如半胱氨酸）诱导氨基酸。总之，诱导碳源与无机磷酸盐、无机氮或硫酸镁的组合是黑曲霉分生孢子发芽的最低要求。