Sodium p-perfluorous nonenoxybenzene sulfonate (OBS), a novel alternative to perfluorooctanesulfonate (PFOS), is widely used in industry as a surfactant, firefighting foam and photographic material. The occurrence of OBS in the aquatic environment has been recently reported, but little information is available on its accumulation and toxic effects in aquatic organisms. In this study, zebrafish larvae (3 d post-fertilization) were subjected to OBS (10, 100 μg/L) and PFOS (10 μg/L) for a period of 48 h, followed by a 24 h of depuration period. The bioconcentration and depuration kinetics, oxidative stress and possible molecular mechanisms of OBS and PFOS were investigated in zebrafish larvae. Our results showed that the uptake and depuration of both OBS and PFOS fitted well with a first-order kinetic model. The uptake rate constant of OBS was similar to that of PFOS, but the depuration rate constant was much higher than PFOS with a half-life of 69.7-85 h for OBS and 222.2 h for PFOS. The calculated BCFs of OBS and PFOS were 238.0-242.5 and 644.2, respectively. In our acute toxicity assay, the enhanced expression of Nrf2 protein accompanied by the upregulation of CAT and SOD protein expressions indicated OBS and PFOS induced oxidative stress in zebrafish larvae, and the Nrf2-ARE signaling pathway was involved in this process. Collectively, OBS has a lower bioconcentration potential than PFOS, but its toxic effect on oxidative stress was comparable to PFOS in zebrafish larvae.

钠p-全氟壬氧基苯磺酸盐（OBS）是全氟辛烷磺酸盐（PFOS）的一种新型替代品，在工业上被广泛用作表面活性剂，灭火泡沫和照相材料。最近报道了OBS在水生环境中的发生，但是关于其在水生生物中的积累和毒性作用的信息很少。在这项研究中，将斑马鱼幼虫（受精后3天）置于OBS（10，100μg/ L）和PFOS（10μg/ L）中48 h，然后进行24 h净化。研究了斑马鱼幼虫中OBS和PFOS的生物浓缩和净化动力学，氧化应激以及可能的分子机制。我们的结果表明，OBS和PFOS的摄取和净化与一阶动力学模型非常吻合。OBS的吸收速率常数与PFOS相似，但是其净化速率常数远高于PFOS，OBS的半衰期为69.7-85 h，PFOS的半衰期为222.2 h。OBS和PFOS的BCF计算值分别为238.0-242.5和644.2。在我们的急性毒性试验中，Nrf2蛋白的表达增强并伴有CAT和SOD蛋白表达的上调，表明OBS和PFOS诱导了斑马鱼幼虫的氧化应激，并且Nrf2-ARE信号通路参与了这一过程。总的来说，OBS具有比PFOS更低的生物富集潜力，但它对氧化应激的毒性作用与斑马鱼幼虫中的PFOS相当。0-242.5和644.2。在我们的急性毒性试验中，Nrf2蛋白的表达增强并伴有CAT和SOD蛋白表达的上调，表明OBS和PFOS诱导了斑马鱼幼虫的氧化应激，并且Nrf2-ARE信号通路参与了这一过程。总的来说，OBS具有比PFOS更低的生物富集潜力，但它对氧化应激的毒性作用与斑马鱼幼虫中的PFOS相当。0-242.5和644.2。在我们的急性毒性试验中，Nrf2蛋白的表达增强并伴有CAT和SOD蛋白表达的上调，表明OBS和PFOS诱导了斑马鱼幼虫的氧化应激，并且Nrf2-ARE信号通路参与了这一过程。总的来说，OBS具有比PFOS更低的生物富集潜力，但它对氧化应激的毒性作用与斑马鱼幼虫中的PFOS相当。