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Isolation, structure identification and hepatoprotective activity of a polysaccharide from Sabia parviflora
Bioorganic & Medicinal Chemistry Letters ( IF 2.7 ) Pub Date : 2020-11-27 , DOI: 10.1016/j.bmcl.2020.127719
Xue-Xue Zhang 1 , Jian-Qiao Li 2 , Man-Shu Li 1 , Yu Chen 1 , Hai-Bo He 1 , Shi-Ping Liu 1 , Fan Cheng 1 , Cheng-Xiong Liu 1 , Kun Zou 1
Affiliation  

The extraction, purification, structure and hepatoprotective activity of a homogenous polysaccharide (SPS60) from Sabia parviflora were investigated. SPS60 was screened after purification with Sephadex G-100 and showed the excellent hepatoprotective activity. Its structural characteristics were investigated by Time of flight mass spectrometry (TOF-MS), PMP Pre-column derivatization-HPLC (PMP-HPLC), nuclear magnetic resonance (NMR) spectroscopy and Atomic Force Microscopy (AFM). The results showed that SPS60 possessed the molecular weight of 16900 Da and the monosaccharide component was glucose, as well as a 1 → 6 glycosidic bond. The results of atomic force microscopy (AFM) show that SPS60 is a blocky sphere in solution. Furthermore, the SPS60 could significantly improve the survival rate of LO2 hepatocytes which were damaged by CCl4. Therefore, SPS60 may be an active substance of S. parviflora as a local functional tea.



中文翻译:

一种小花椴多糖的分离、结构鉴定及保肝活性

从均匀的多糖(SPS60)的提取,纯化,结构和保肝活性萨比亚小花进行了调查SPS60 经 Sephadex G-100 纯化后筛选,显示出优异的保肝活性。通过飞行时间质谱(TOF-MS)、PMP柱前衍生化-HPLC(PMP-HPLC)、核磁共振(NMR)光谱和原子力显微镜(AFM)对其结构特征进行了研究。结果表明,SPS60的分子量为16900 Da,单糖成分为葡萄糖,并具有1→6个糖苷键。原子力显微镜 (AFM) 的结果表明 SPS60 在溶液中是块状球体。此外,SPS60可以显着提高被CCl 4损伤的LO2肝细胞的存活率。因此,SPS60 可能是S. parviflora作为功​​能性茶点的活性物质。

更新日期:2020-12-02
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