Relapse and metastasis of tumor may occur for osteosarcoma (OS) patients after clinical resection. Conventional metallic scaffolds provide sufficient mechanical support to the defected bone but fail to eradicate recurring tumors. Here we report that biodegradable magnesium (Mg) wire-based implant can inhibit OS growth. In brief, the Mg wires release Mg ions to activate the transport of zinc finger protein Snail1 from cytoplasm to cell nucleus, which induces apoptosis and inhibits proliferation of OS cells through a parallel antitumor signaling pathway of miRNA-181d-5p/TIMP3 and miRNA-181c-5p/NLK downstream. Simultaneously, the hydrogen gas evolution from Mg wires eliminates intracellular excessive reactive oxygen species, by which the growth of bone tumor cells is suppressed. The subcutaneous tumor-bearing experiment of OS cells in nude mice further confirms that Mg wires can effectively inhibit the growth of tumors and prolong the survival of tumor-bearing mice. In addition, Mg wires have no toxicity to normal cells and tissues. These results suggest that Mg implant is a potential anti-tumor scaffold for OS patients.

临床切除后的骨肉瘤（OS）患者可能会发生肿瘤复发和转移。常规的金属支架为缺损的骨提供足够的机械支撑，但是不能根除复发的肿瘤。在这里我们报告生物可降解的镁（Mg）丝为基础的植入物可以抑制OS的增长。简而言之，Mg线释放Mg离子以激活锌指蛋白Snail1从细胞质到细胞核的转运，从而通过miRNA-181d-5p / TIMP3和miRNA-的平行抗肿瘤信号通路诱导凋亡并抑制OS细胞的增殖。下游为181c-5p / NLK。同时，由Mg丝释放出的氢气消除了细胞内过量的活性氧，从而抑制了骨肿瘤细胞的生长。裸鼠中OS细胞的皮下荷瘤实验进一步证实，Mg线可有效抑制肿瘤的生长并延长荷瘤小鼠的生存期。另外，镁丝对正常细胞和组织没有毒性。这些结果表明，Mg植入物是OS患者潜在的抗肿瘤支架。