In this study, tansy (Tanacetum vulgare L.) in vitro culture was established from seeds collected from natural populations. The multiplication of plantlets was conducted through shoot tips that exhibited potent apical growth and regeneration capacities on basal medium (BM), without the addition of any plant growth regulators (PGRs). PGRs were also omitted for the establishment and cultivation of tansy root cultures. Both abaxial and adaxial leaf surfaces of in vitro micropropagated plantlets were covered with glandular biseriate trichomes. Histochemical staining showed that glandular secretions were rich in lipid and terpene compounds, confirmed by GC-MS analysis of essential oil (EO). In the total EO, similar portions of oxygenated monoterpenes (38.5% m/m) and oxygenated sesquiterpenes (22.6% m/m) were detected. Chemical profiles of methanol extracts of in vitro cultured tansy shoots and roots varied in quantity and quality from those obtained from wild-growingtansy. HPLC analysis indicated that the methanol extracts of in vitro cultured roots were the richest in 3,5-O-dicaffeoylquinic acid (3,5-O-DCQA), in which the concentration was 6 times higher (10.220 mg/g DW) than that in the extract obtained from roots of wild-growing tansy (1.684 mg/g DW). This result is noticeable in the manner of industrial production of biologically active 3,5-O-DCQA that has been shown to have antioxidant, hepatoprotective, antiviral, antimutagenic, and immunomodulatory activity. Biotechnological interventions on secondary metabolite production taking place in trichomes could further enhance the production of some important tansy metabolites and further investigation will be directed toward the elucidation of the pharmaceutical potential of tansy in vitro obtained metabolites, as mixtures or single moieties.

中文翻译：

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艾菊（Tanacetum vulgare L.）的体外培养：生产强效药剂的工具


在这项研究中，艾菊 (Tanacetum vulgare L.) 的体外培养物是从自然种群中收集的种子建立的。幼苗的繁殖是通过茎尖进行的，在基础培养基（BM）上表现出强大的顶端生长和再生能力，无需添加任何植物生长调节剂（PGR）。艾菊根培养物的建立和培养也省略了 PGR。体外微繁殖植株的叶背面和叶表面均覆盖有腺双丝状毛。组织化学染色显示，腺体分泌物富含脂质和萜烯化合物，精油 (EO) 的 GC-MS 分析证实了这一点。在总 EO 中，检测到相似部分的含氧单萜 (38.5% m/m) 和含氧倍半萜 (22.6% m/m)。体外培养的艾菊芽和根的甲醇提取物的化学特征在数量和质量上与从野生艾菊中获得的那些不同。 HPLC分析表明，体外培养的根的甲醇提取物中3,5-O-二咖啡酰奎尼酸（3,5-O-DCQA）含量最高，其浓度比对照高6倍（10.220 mg/g DW）。取自野生艾菊根部的提取物（1.684 mg/g DW）。这一结果在生物活性 3,5-O-DCQA 的工业生产方式中引人注目，该物质已被证明具有抗氧化、保肝、抗病毒、抗诱变和免疫调节活性。 对毛状体中发生的次级代谢产物产生的生物技术干预可以进一步增强一些重要的艾菊代谢物的产生，并且进一步的研究将旨在阐明艾菊体外获得的代谢物（作为混合物或单一部分）的药物潜力。