Introduction

A large scale population exposure to ionizing radiation during intentional or unintentional nuclear accidents undoubtedly generates a complex scenario with partial-body as well as total-body irradiated victims. A high throughput technique based rapid assessment method is an urgent necessity for stratification of exposed subjects independent of whether exposure is uniform total-body or non-homogenous partial-body.

Objective

Here, we used Nuclear Magnetic Resonance (NMR) based metabolomics approach to compare and identify candidate metabolites differentially expressed in total and partially irradiated mice model.

Methods

C57BL/6 male mice (8–10 weeks) were irradiated total-body or locally to thoracic, hind limb or abdominal regions with 10 Gy of gamma radiation. Urine samples collected at 24 h post irradiation were examined using high resolution NMR spectroscopy and the datasets were analysed using multivariate analysis.

Results

Multivariate and metabolic pathway analysis in urine samples collected at 24 h post-radiation exhibited segregation of all irradiated groups from controls. Metabolites associated with energy metabolism, gut flora metabolism and taurine were common to partial and total-body irradiation, thus making them potential candidates for radiation exposure. Nevertheless, a distinct metabolic pattern was observed in partial-body exposed groups with maximum changes observed in the hind limb region indicating differential tissue associated radiation sensitivity. The organ-specific changes may provide an early warning regarding the physiological system at risk after radiation injury.

Conclusion

The study affirms potentiality of metabolite markers and comparative analysis could be an important piece of information for an integrated solution to a complex research question in terms of radiation biomarkers.

中文翻译：

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使用非靶向代谢组学方法比较小鼠全身和部分辐射暴露的代谢特征

介绍

在有意或无意的核事故中大规模暴露于电离辐射的人群无疑会产生一个复杂的场景，既有局部辐射受害者，也有全身辐射受害者。一种基于高通量技术的快速评估方法是对暴露对象进行分层的迫切需要，而不管暴露是均匀的全身暴露还是非均匀的局部暴露。

客观的

在这里，我们使用基于核磁共振 (NMR) 的代谢组学方法来比较和识别在全部和部分辐照小鼠模型中差异表达的候选代谢物。

方法

C57BL/6 雄性小鼠（8-10 周）接受全身或局部胸部、后肢或腹部区域的 10 Gy 伽马辐射照射。使用高分辨率 NMR 光谱检查在照射后 24 小时收集的尿液样本，并使用多变量分析对数据集进行分析。

结果

在辐射后 24 小时收集的尿液样本中的多变量和代谢途径分析显示所有受辐射组与对照组的分离。与能量代谢、肠道菌群代谢和牛磺酸相关的代谢物在局部和全身照射中很常见，因此使它们成为辐射暴露的潜在候选者。然而，在部分身体暴露组中观察到明显的代谢模式，在后肢区域观察到最大变化，表明与辐射敏感性相关的不同组织。器官特异性变化可以提供关于辐射损伤后处于危险中的生理系统的早期警告。

结论

该研究肯定了代谢物标志物的潜力，比较分析可能是综合解决辐射生物标志物方面复杂研究问题的重要信息。