Rosellinia necatrix Prill. the causal agent of white root rot diseases causes major losses in avocado crops in Spain. To study the biocontrol of this disease, 31 antagonistic isolates were collected from the avocado rhizosphere and identified as Entoleuca sp. Studies were carried out in mycovirus detection, in vitro optimal growth temperature, mycelial compatibility groups of these isolates, and their inhibition of R. necatrix growth. The pathogenicity of these antagonistic isolates and their biocontrol of white root rot on avocado plants were also studied. The presence of mycoviruses was detected in all 31 Entoleuca sp. isolates studied and the optimal growth temperature for almost all the isolates (> 80%) was 25 °C. Dual culture showed different responses, with some isolates compatible and others incompatible with each other, showing inhibition halos and black lines. Twenty-five of the 31 isolates showed significant growth inhibition to a virulent R. necatrix isolate in in vitro conditions. In addition, none of the Entoleuca sp. isolates produced any symptoms on inoculated avocado plants and in 24 of them, symptoms of avocado white root rot were reduced, with significant differences among the Entoleuca sp. isolates when they were used as biocontrol agents. New virus-carrying Entoleuca sp. isolates were obtained, which are capable of controlling avocado white root rot.

玫瑰茄necatrix造粒。白根腐烂病的病原体在西班牙造成鳄梨作物的重大损失。为了研究这种疾病的生物防治，从鳄梨根际中收集了31种拮抗菌株，并将其鉴定为Entoleuca sp。研究进行了分枝杆菌病毒检测，体外最佳生长温度，这些分离物的菌丝体相容性基团及其对中性鼻螺旋体生长的抑制作用。还研究了这些拮抗菌株的致病性及其对鳄梨植物白根腐病的防治作用。在所有31个Entoleuca中均检测到了分枝杆菌病毒的存在sp。对分离株进行了研究，几乎所有分离株（> 80％）的最佳生长温度均为25°C。双重培养显示出不同的反应，某些分离株相容，另一些彼此不相容，表现出抑制晕圈和黑线。31种分离株中有25种在体外条件下显示出对有毒的R. necatrix分离株的显着生长抑制作用。此外，没有Entoleuca sp。分离株在接种的鳄梨植物上未产生任何症状，其中24种鳄梨白根腐烂症状有所减轻，Entoleuca sp。之间存在显着差异。用作生物防治剂时分离。新型携带病毒的Entoleucasp。获得了能够控制鳄梨白根腐烂的分离株。