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The morphological effect of nanostructured hydroxyapatite coatings on the osteoinduction and osteogenic capacity of porous titanium
Nanoscale ( IF 5.8 ) Pub Date : 2020-11-12 , DOI: 10.1039/d0nr06306a Minxun Lu 1, 2, 3, 4, 5 , Hongjie Chen 3, 4, 5, 6 , Bo Yuan 3, 4, 5, 6 , Yong Zhou 1, 2, 3, 4, 5 , Li Min 1, 2, 3, 4, 5 , Zhanwen Xiao 3, 4, 5, 6 , Xiao Yang 3, 4, 5, 6 , Xiangdong Zhu 3, 4, 5, 6 , Chongqi Tu 1, 2, 3, 4, 5 , Xingdong Zhang 3, 4, 5, 6
Nanoscale ( IF 5.8 ) Pub Date : 2020-11-12 , DOI: 10.1039/d0nr06306a Minxun Lu 1, 2, 3, 4, 5 , Hongjie Chen 3, 4, 5, 6 , Bo Yuan 3, 4, 5, 6 , Yong Zhou 1, 2, 3, 4, 5 , Li Min 1, 2, 3, 4, 5 , Zhanwen Xiao 3, 4, 5, 6 , Xiao Yang 3, 4, 5, 6 , Xiangdong Zhu 3, 4, 5, 6 , Chongqi Tu 1, 2, 3, 4, 5 , Xingdong Zhang 3, 4, 5, 6
Affiliation
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Weak osteogenic activity affects the long-term fixation and lifespan of titanium (Ti) implants. Surface modification along with a built-in porous structure is a highly considerable approach to improve the osteoinduction and osseointegration capacity of Ti. Herein, the osteoinduction and osteogenic activities of electrochemically deposited (ED) nanoplate-like, nanorod-like and nanoneedle-like hydroxyapatite (HA) coatings (named EDHA-P, EDHA-R, and EDHA-N, respectively) were evaluated in vitro and in vivo by comparison with those of acid/alkali (AA) treatment. The results revealed that the apatite forming ability of all nanostructured EDHA coatings was excellent, and only 12 h of soaking in SBF was needed to induce a complete layer of apatite. More serum proteins adsorbed on EDHA-P than others. In cellular experiments, different from those on EDHA-R and EDHA-N, the cells on EDHA-P presented a polygonal shape with lamellipodia extension, and thus exhibited a relatively larger spreading area. Furthermore, EDHA-P was more favorable for the enhancement of the proliferation and ALP activity of BMSCs, and the up-regulation of OPN gene expression. Based on the good biological performance in vitro, EDHA-P was selected to further evaluate its osteoinduction and osteogenic activities in vivo by comparison with AA treatment. Interestingly, a greater ability of ectopic osteoinduction was observed in the EDHA-P group compared to that in the AA group. At the osseous site, EDHA-P promoted more bone on/ingrowth, and had a higher area percentage of newly formed bone in the bone–implant interface and inner pores of the implants than in the AA group. Thus, a nanoplate-like HA coating has good potential in improving the osteoinductivity and osteogenic activity of porous Ti implants in clinical applications.
中文翻译：
纳米羟基磷灰石涂层的形态对多孔钛骨诱导和成骨能力的影响
成骨活性弱会影响钛（Ti）植入物的长期固定和寿命。表面修饰以及内置的多孔结构是提高Ti的骨诱导和骨整合能力的一种非常重要的方法。在这里，体外评估电化学沉积（ED）纳米板状，纳米棒状和纳米针状羟基磷灰石（HA）涂层（分别命名为EDHA-P，EDHA-R和EDHA-N）的骨诱导和成骨活性。和体内与酸/碱（AA）处理相比。结果表明，所有纳米结构EDHA涂层的磷灰石形成能力都非常好，只需在SBF中浸泡12小时即可诱导出完整的磷灰石层。EDHA-P上吸附的血清蛋白比其他更多。在细胞实验中，与EDHA-R和EDHA-N上的细胞不同，EDHA-P上的细胞呈带片状脂膜延伸的多边形，因此展布了相对较大的面积。此外，EDHA-P更有利于增强BMSCs的增殖和ALP活性，以及上调OPN基因表达。基于体外良好的生物学性能，选择EDHA-P进一步评估其体内的骨诱导和成骨活性与AA治疗相比。有趣的是，与AA组相比，EDHA-P组的异位骨诱导能力更高。与AA组相比，EDHA-P在骨部位促进了更多的骨骼生长/向内生长，并且在骨-植入物界面和植入物的内孔中新形成的骨骼的面积百分比更高。因此，纳米板状HA涂层在临床应用中具有改善多孔Ti植入物的骨诱导性和成骨活性的良好潜力。
更新日期：2020-11-27
中文翻译：
纳米羟基磷灰石涂层的形态对多孔钛骨诱导和成骨能力的影响
成骨活性弱会影响钛（Ti）植入物的长期固定和寿命。表面修饰以及内置的多孔结构是提高Ti的骨诱导和骨整合能力的一种非常重要的方法。在这里，体外评估电化学沉积（ED）纳米板状，纳米棒状和纳米针状羟基磷灰石（HA）涂层（分别命名为EDHA-P，EDHA-R和EDHA-N）的骨诱导和成骨活性。和体内与酸/碱（AA）处理相比。结果表明，所有纳米结构EDHA涂层的磷灰石形成能力都非常好，只需在SBF中浸泡12小时即可诱导出完整的磷灰石层。EDHA-P上吸附的血清蛋白比其他更多。在细胞实验中，与EDHA-R和EDHA-N上的细胞不同，EDHA-P上的细胞呈带片状脂膜延伸的多边形，因此展布了相对较大的面积。此外，EDHA-P更有利于增强BMSCs的增殖和ALP活性，以及上调OPN基因表达。基于体外良好的生物学性能，选择EDHA-P进一步评估其体内的骨诱导和成骨活性与AA治疗相比。有趣的是，与AA组相比，EDHA-P组的异位骨诱导能力更高。与AA组相比，EDHA-P在骨部位促进了更多的骨骼生长/向内生长，并且在骨-植入物界面和植入物的内孔中新形成的骨骼的面积百分比更高。因此，纳米板状HA涂层在临床应用中具有改善多孔Ti植入物的骨诱导性和成骨活性的良好潜力。
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