A liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method has been validated for the simultaneous determination of methamphetamine (MA) and 3,4-methylenedioxy-N-methamphetamine (MDMA) in the blood sample. Under the optimal experimental conditions, the concentration of MA can be determined in the range from 1 µg/L to 5000 µg/L with the method detection limit (MDL) of 0.31 µg/L. The range from 0.5 to 500 µg/L is observed for the determination of MDMA with the MDL down to 0.25 µg/L. The practical applicability of the method is performed with the recovery ranging from 85.3% to 94% for MA and from 86.9% to 95.5% for MDMA. At the different concentrations of drugs, the relative standard deviations (RSD) for both MA and MDMA are lower than 5.7%. The method was applied to analyse 1995 blood samples that had been collected from the Forensic Medicine Centre of Ho Chi Minh City. The results showed 1.75% positive with MA and 0.25% positive with MDMA. These two drugs take 10% of the total drugs positive samples. By using deuterium-labelled methamphetamine-d5 and 3,4-methylenedioxy-N-methamphetamine-d5 as the internal standards in the determination and the use of MS/MS in multiple reaction monitoring mode signal readout, the method exhibits robustness specificity and can be applied in simultaneous determination of MA and MDMA in blood with high selectivity and sensitivity.

中文翻译：

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一种同时测定血基液相色谱-串联质谱中甲基苯丙胺和3,4-亚甲二氧基-N-甲基苯丙胺的有效方法

液相色谱-串联质谱法（HPLC-MS / MS）已被验证用于同时测定血样中的甲基苯丙胺（MA）和3,4-亚甲二氧基-N-甲基苯丙胺（MDMA）。在最佳实验条件下，MA的浓度可以在1 µg / L至5000  µg / L的范围内测定， 方法检测限（MDL）为0.31  µg / L。从0.5到500的范围内 μ g / L的用于MDMA的确定与MDL观察下降到0.25  μ克/升 该方法的实际适用性以MA的回收率为85.3％至94％，MDMA的回收率为86.9％至95.5％。在药物的不同浓度下，MA和MDMA的相对标准偏差（RSD）均低于5.7％。该方法用于分析从胡志明市法医中心收集的1995年血液样本。结果显示MA阳性1.75％，MDMA阳性0.25％。这两种药物占全部药物阳性样本的10％。通过使用氘标记的甲基苯丙胺-d5和3,4-亚甲基二氧基-N-甲基苯丙胺-d5作为内标，在多反应监测模式信号读出中使用MS / MS进行测定，