Abstract Mosquito-borne diseases are causing serious damage to public health worldwide, and control of these deadly mosquito vectors is a major thrust area for epidemiologists and public health workers. Therefore, the present research reports an eco-friendly solution with multipotency of silver nanoparticle fabricated from shrimp shell biowaste in controlling mosquitoes and bacterial pathogens. The biofabricated chitosan silver nanoparticles (Cs-AgNPs) were confirmed by UV-visible spectroscopy, transmission electron microscopy (TEM), Fourier transform infrared spectroscopy, energy dispersive X-ray spectroscopy, X-ray powder diffraction and zeta potential analysis. The TEM studies showed that the obtained Cs-AgNPs were mostly spherical in shape. Low doses of chitosan and Cs-AgNPs showed high mosquitocidal properties against both larvae and adult of Anopheles stephensi, Aedes aegypti, and Culex quinquefasciatus. The LC50 (lethal concentration 50%) of Cs-AgNPs was 10.240 ppm (fourth instar larvae) and 9.671 ppm (adult) for An. stephensi; 11.349 ppm (fourth instar) and 12.015 ppm (adult) for Ae. aegypti and 12.426 ppm (fourth instar) and 12.965 ppm (adult) for Cx. quinquefasciatus. The concerning part of antibacterial studies showed that Cs-AgNP had significant inhibition on tested bacterial pathogens. Overall, this study shows that chitosan extracted from the shrimp shell wastes can be used as a potential source for controlling major mosquito vectors.

中文翻译：

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来自虾壳废物的壳聚糖银纳米粒子对具有公共卫生重要性的主要蚊媒的功效

摘要 蚊媒疾病正在对全世界的公共卫生造成严重损害，控制这些致命的蚊媒是流行病学家和公共卫生工作者的主要重点领域。因此，本研究报告了一种生态友好的解决方案，该解决方案具有由虾壳生物废物制成的银纳米颗粒的多能性，可用于控制蚊子和细菌病原体。生物制造的壳聚糖银纳米粒子 (Cs-AgNPs) 通过紫外可见光谱、透射电子显微镜 (TEM)、傅里叶变换红外光谱、能量色散 X 射线光谱、X 射线粉末衍射和 zeta 电位分析得到证实。TEM 研究表明，获得的 Cs-AgNPs 大多呈球形。低剂量的壳聚糖和 Cs-AgNPs 对史蒂芬斯按蚊、埃及伊蚊和库蚊的幼虫和成虫均表现出很高的杀蚊特性。Cs-AgNPs 的 LC50（致死浓度为 50%）为 10.240 ppm（四龄幼虫）和 9.671 ppm（成虫）。斯蒂芬西; Ae 为 11.349 ppm（四龄）和 12.015 ppm（成人）。aegypti 和 12.426 ppm（四龄）和 12.965 ppm（成人）Cx。五花肉。抗菌研究的相关部分表明，Cs-AgNP 对测试的细菌病原体有显着的抑制作用。总的来说，这项研究表明，从虾壳废物中提取的壳聚糖可用作控制主要蚊子媒介的潜在来源。An 为 240 ppm（四龄幼虫）和 9.671 ppm（成虫）。斯蒂芬西; Ae 为 11.349 ppm（四龄）和 12.015 ppm（成人）。aegypti 和 12.426 ppm（四龄）和 12.965 ppm（成人）Cx。五花肉。抗菌研究的相关部分表明，Cs-AgNP 对测试的细菌病原体有显着的抑制作用。总的来说，这项研究表明，从虾壳废物中提取的壳聚糖可用作控制主要蚊子媒介的潜在来源。An 为 240 ppm（四龄幼虫）和 9.671 ppm（成虫）。斯蒂芬西; Ae 为 11.349 ppm（四龄）和 12.015 ppm（成人）。aegypti 和 12.426 ppm（四龄）和 12.965 ppm（成人）Cx。五花肉。抗菌研究的相关部分表明，Cs-AgNP 对测试的细菌病原体有显着的抑制作用。总的来说，这项研究表明，从虾壳废物中提取的壳聚糖可用作控制主要蚊子媒介的潜在来源。