Onychomycosis is a disease that affects many adults, whose treatment includes both oral and topical therapies with low cure rates. The topical therapy is less effective but causes fewer side effects. This is why the development of an effective, easy to apply formulation for topical treatment is of high importance. We have used a nanotechnological approach to formulate Pickering emulsions (PEs) with well-defined properties to achieve site-specific delivery for antifungal drug combination of tioconazole and Melaleuca alternifolia essential oil. Silica nanoparticles with tailored size and partially hydrophobic surface have been synthesized and used for the stabilization of PEs. In vitro diffusion studies have been performed to evaluate the drug delivery properties of PEs. Ethanolic solution (ES) and conventional emulsions (CE) have been used as reference drug formulations. The examination of the antifungal effect of PEs has been performed on Candida albicans and Trichophyton rubrum as main pathogens. In vitro microbiological experimental results suggest that PEs are better candidates for onychomycosis topical treatment than CE or ES of the examined drugs. The used drugs have shown a significant synergistic effect, and the combination with an effective drug delivery system can result in a promising drug form for the topical treatment of onychomycosis.

中文翻译：

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用于甲真菌病局部治疗的噻康唑和互叶白千层精油 Pickering 乳液的配方

灰指甲是一种影响许多成年人的疾病，其治疗包括口服和局部治疗，治愈率低。局部治疗效果较差，但副作用较少。这就是为什么开发一种有效、易于应用的局部治疗制剂非常重要。我们使用纳米技术方法配制具有明确特性的 Pickering 乳液 (PE)，以实现特定部位递送噻康唑和互叶白千层精油的抗真菌药物组合。具有定制尺寸和部分疏水表面的二氧化硅纳米粒子已被合成并用于稳定 PE。已经进行了体外扩散研究以评估 PE 的药物递送特性。乙醇溶液 (ES) 和常规乳液 (CE) 已被用作参考药物制剂。已对作为主要病原体的白色念珠菌和红色毛癣菌进行了 PE 抗真菌作用的检验。体外微生物实验结果表明，PEs 比 CE 或 ES 更适合用于甲真菌病局部治疗。所使用的药物显示出显着的协同作用，与有效的药物递送系统相结合，可以产生一种有前景的用于局部治疗甲真菌病的药物形式。体外微生物实验结果表明，PEs 比 CE 或 ES 更适合用于甲真菌病局部治疗。所使用的药物显示出显着的协同作用，与有效的药物递送系统相结合，可以产生一种有前景的用于局部治疗甲真菌病的药物形式。体外微生物实验结果表明，PEs 比 CE 或 ES 更适合用于甲真菌病局部治疗。所使用的药物显示出显着的协同作用，与有效的药物递送系统相结合，可以产生一种有前景的用于局部治疗甲真菌病的药物形式。